At baseline, blood samples were obtained, centrifuged, and frozen at -80°C in 1 ml aliquots until the time of analysis. The samples of 1178 subjects with available serum were shipped on dry ice to the Department of Clinical Chemistry, University Hospital of Zurich, for biomarker measurements. Samples were thawed at 4 °C prior to measurements and analyzed within one hour, thus reducing pre-analytic errors. PCT concentrations were measured using a sensitive assay with a detection limit of 0.007 ng/ml (B·R·A·H·M·S-us PCT sensitive KRYPTOR, Thermo-Scientific, BRAHMS, Germany). MR-proANP concentrations were measured using an immunoassay with a detection limit of 2.1 pmol/L (B·R·A·H·M·S KRYPTOR, Thermo Scientific, BRAHMS, Germany). Quality control was maintained using standardized procedures. All testing was performed in a batched analysis, blinded to all clinical data. Stability at room temperature and after freezing and thawing cycles has been documented for both biomarkers 18 (link), 19 (link).
Kryptor
Manufactured by Thermo Fisher Scientific
Sourced in Germany
The KRYPTOR is a compact, automated immunoassay analyzer designed for the quantitative determination of various analytes in biological samples. It utilizes fluorescence detection technology to perform high-throughput immunoassays. The KRYPTOR is capable of running a wide range of assays, including those for therapeutic drug monitoring, infectious diseases, and endocrinology.
Automatically generated - may contain errors
15 protocols using kryptor
1
Biomarker Measurements in Serum Samples
2
Chromogranin A Quantification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CGA levels were measured on the fully automated B.R.A.H.M.S KRYPTOR instrument (Thermo Scientific B.R.A.H.M.S GmbH, Hennigsdorf/Berlin, Germany) using the B.R.A.H.M.S. CGA II homogeneous sandwich fluoroimmuno-assay as described previously (20 (link), 21 (link), 22 (link)). The functional sensitivity of this assay is 13.7 ng/mL. Two commercial CGA controls were measured in each assay. The quality controls mean intra-assay variabilities for duplicate measurements were 2.7 and 1.4%, while the inter-assay variabilities were 4.7 and 3.4%.
3
Biomarker Quantification in STEMI Patients
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Heparinized blood samples were collected from all patients 4 months following STEMI by peripheral venipuncture. Samples for NT-proBNP were promptly analysed at the central laboratory of the University Hospital of Innsbruck by personnel blinded to study data. MR-proANP and MR-proADM were measured in batches after storage at −80°C. Assays used for the determination of NT-proBNP, MR-proANP and MR-proADM have previously been described [19 (link), 21 (link)]. Briefly, NT-proBNP concentrations were measured using a commercially available assay with an E170 instrument (proBNP II assay using monoclonal antibodies on a Modular, Roche Diagnostics, Vienna, Austria). The analytical limit of detection of NT-proBNP is 5 ng/L and the limit of quantification is 50 ng/L. The intra-assay coefficient of variations (CV) are 1.9% at a concentration of 64 ng/L and 1.2% at a concentration of 2105 ng/L, and the inter-assay CVs are 3.1% at a concentration of 46 ng/L and 2.7% at a concentration of 2170 ng/L according to the package insert. MR-proANP and MR-proADM were measured by fully automated fluorescence immunoassays (Kryptor, Thermo Fisher Scientific, B.R.A.H.M.S., Hennigsdorf, Germany). The analytical limit of detection of MR-proANP is 0.05 nmol/L and the limit of quantification 0.23 nmol/L. The limit of detection for the MR-proADM assay is 2.1 pmol/L and the limit of quantification is 4.5 pmol/L.
4
Copeptin Biomarker Assessment in COVID-19
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Following hospital admission, plasma and serum samples were collected in BD Vacutainer Heparin and serum-separating tube (SST) tubes, and leftover samples were stored at -80°C until analysis. Results from routine laboratory tests were recorded. Copeptin was assessed in batch using a commercially available automated fluorescent sandwich immunoassay (BRAHMS KRYPTOR; Thermo Fisher Scientific, Hennigsdorf, Germany), as described in detail elsewhere [15 (link), 16 (link)]. The immunoassay has a limit of detection of 0.9 pmol/L, and the functional assay sensitivity—defined as concentration with an interassay coefficient of variation of <20%—was 2.0 pmol/L. Values for the analytes followed Gaussian distribution in healthy individuals without significant difference between males and females, as listed in the assay documentation. Laboratory technicians measuring copeptin were blinded to patient characteristics and study details.
For the SARS-CoV-2-affected patients, different timepoints during hospitalization were analyzed, namely T0 (initial blood draw upon hospital admission), T1 (day 3/4), T2 (day 5/6), and T3 (day 7/8). For the control group, blood samples for biomarker measurement were upon hospital admission. Copeptin levels for these patients were batch-measured in plasma with sandwich immunoassays (KRYPTOR; Thermo Scientific) [13 (link)].
For the SARS-CoV-2-affected patients, different timepoints during hospitalization were analyzed, namely T0 (initial blood draw upon hospital admission), T1 (day 3/4), T2 (day 5/6), and T3 (day 7/8). For the control group, blood samples for biomarker measurement were upon hospital admission. Copeptin levels for these patients were batch-measured in plasma with sandwich immunoassays (KRYPTOR; Thermo Scientific) [13 (link)].
5
PCT Measurement Using KRYPTOR Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Currently, the B·R·A·H·M·S PCT sensitive KRYPTOR assay (Thermo Scientific, Hennigsdorf, Germany) is considered the “gold standard” for PCT measurements in a clinical setting, and has been used to develop clinical cut-offs and algorithms. The KRYPTOR random access analyzer for homogeneous immunoassays in human serum or plasma uses the time-resolved amplified cryptate emission (TRACE) technology. It has a functional assay sensitivity (LLoQ) for PCT of 0.06 μg·L−1. All KRYPTOR measurements were performed at the Central Laboratory of University of Tübingen Medical Center. The Laboratory holds an accreditation according to ISO 15189, and internal as well as external quality controls for PCT were within the allowed ranges at all times.
6
Evaluation of MR-proADM in ED Patients
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Measurement of MR-proADM levels was performed on EDTA (Ethylenediaminetetraacetic acid) blood samples within 48 h of being taken on evaluation in ED (in line with manufacturer’s guidance stating a 72 h period of stability) using an immunoassay (B.R.A.H.M.S. KRYPTOR™, Thermo Fisher Scientific, Henningsdorf, Germany). Data collected included demographics, prior comorbidities, clinical outcomes such as admission and mortality at 28 days. Blood results including White Blood Cell Count (WBC), lymphocyte count, C-reactive Protein (CRP), Procalcitonin (PCT), lactate dehydrogenase (LDH), D-dimer measurements and the raw data to calculate clinical scores like NEWS2 and SOFA, were collected when these were performed at presentation to ED. All samples were analysed as per each site’s laboratory procedures.
7
Procalcitonin Measurement in Clinical Care
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood cell count and C-reactive protein (CRP) analysis was performed as part of routine clinical care. Procalcitonin (PCT) testing was performed retrospectively in a batched analysis and results were not available to treating physicians. PCT levels were measured using a sensitive assay with a detection limit of 0.007 ng/mL (B·R·A·H·M·S PCT sensitive KRYPTOR, Thermo-Scientific, Berlin, Germany) [20 (link)].
8
Biomarker Profiling in Febrile Illness
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An analytical control (blood count), biochemistry with biomarkers determination, peripheral/central blood cultures, urine (systematic and sediment and culture) and pharyngeal exudate for viruses and bacteria were collected within 12 h from the onset of fever. Residual samples were obtained at 12–24 h, 25–48 h and 49–72h after fever commenced. Blood samples were drawn into tubes containing lithium-heparin as anticoagulant for determination of CRP, PCT and IL-6. An additional tube containing plasma EDTA was also drawn for freezing at −80 °C and further processing of MR-proADM. Plasma CRP was measured in a Cobas 8000/module C501, PCT and IL-6 were measured in a Cobas E601 (Roche Diagnostic, Mannheim, Germany) whereas MR-proADM was measured in a Kryptor® (Thermo Fisher Scientific, Hennigsdorf, Germany). Analytical detection limits were 0.07 mg/dL for CRP, 0.02 ng/mL for PCT, 1.5 pg/mL for IL-6 and 0.08 nmol/L for MR-proADM.
9
Quantification of Plasma Peptide Biomarkers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Absolute levels of stable fragments of the peptide precursors MR-proADM, MR-proANP and copeptin were measured in EDTA-plasma using a standardized, commercial immunoluminometric sandwich assay, as previously described (KRYPTOR, Thermo Fisher Scientific, Hennigsdorf/Berlin, Germany) [17 (link),18 (link),19 (link)].
10
Procalcitonin Measurement for Asthma Exacerbation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Procalcitonin was measured for all subjects in the asthma exacerbation cohort. Serum samples were measured on the Roche Elecsys 2010 analyzer (Roche Diagnostics) or miniVIDAS immunoassay (bioMérieux). Plasma samples were measured using B·R·A·H·M·S Procalcitonin sensitive KRYPTOR (Thermo Fischer Scientific). Values >0.25 μg/liter assigned patients as having a bacterial trigger for asthma, while values ≤0.25 μg/liter were considered non-bacterial[24 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!