Takyon rox probe core kit dttp

Manufactured by Eurogentec

The Takyon Rox probe core kit dTTP is a laboratory equipment product. It is a part of the Takyon product line offered by Eurogentec. The core function of this kit is to provide a reagent solution for use in real-time PCR experiments.

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Lab products found in correlation

Nucleospin rna kit, by Macherey-Nagel (1 mentions) Revertaid kit, by Thermo Fisher Scientific (1 mentions) Tripure reagent, by Roche (1 mentions)

2 protocols using takyon rox probe core kit dttp

RNA Extraction and RT-qPCR Analysis

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Total RNA was extracted from cells with the NucleoSpin RNA kit from Macherey Nagel (#740955) and was retrotranscribed to complementary DNA (cDNA) by using the RevertAid kit from ThermoFisher (#K1691). RT-qPCR experiments were performed with the Takyon Rox probe core kit dTTP from Eurogentec (#UF-RPCT-C0201) in a StepOnePlus thermal cycler (Applied Biosystems). Primers and probes were purchased from Eurogentec. Primer and probe sequences used are listed in online supplemental file 1.

Solvay M., Holfelder P., Klaessens S., Pilotte L., Stroobant V., Lamy J., Naulaerts S., Spillier Q., Frédérick R., De Plaen E., Sers C., Opitz C.A., Van den Eynde B.J, & Zhu J. (2023). Tryptophan depletion sensitizes the AHR pathway by increasing AHR expression and GCN2/LAT1-mediated kynurenine uptake, and potentiates induction of regulatory T lymphocytes. Journal for Immunotherapy of Cancer, 11(6), e006728.

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Characterizing Melanocyte Transcriptome

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RNA was extracted from FACS-sorted cell populations using TRIPURE reagent (Roche). One microgram of total RNA was used to generate cDNA using the revertAid (Thermo Fisher). RT-qPCR was performed using Takyon Rox probe core kit dTTP (Eurogentec). The following primers were used: Trap1a (P1A): forward: 5ʹ-AGC-TGA-GGA-AAT-GGG-TGC-TG-3ʹ (exon 1), reverse: 5ʹ-CAG-CAT-TTT-CAC-ACC-TAC-ACT-CCA-3ʹ (exon 2), probe: 5ʹ-FAM-CCA-TCA-TTT-AAG-GAA-GAA-TGA-AGT-GAA-GTG-TAG-GAT-GA-TAMRA-3ʹ (exon 2). Reactions were performed using an elongation condition of 60°C for 1 min. Probe and primers were purchased from Thermo Fisher. Tgfb1 (Mm01178820_m1); Tgfb2 (Mm00436955_m1); Tgfb3 (Mm00436960_m1); Mitf (Mm00434954_m1); Tyr (Mm00495817_m1); Tyrp1 (Mm004453201_m1); Dct (Mn01225584_m1); Twist (Mm00442036_m1); Snai1 (Mm00441533_g1); Zeb1 (Mm00495564_m1); Zeb2 (Mm00497196_m1); Tgfbi (Mm01337605_m1); Vmac (Mm00619195_g1); Cdh1 (Mm01247357_m1); Cdh2 (Mm 01162497_m1); Acta2 (Mm00725412_g1); Gapdh (Mm99999915_g1).

Canè S., Van Snick J., Uyttenhove C., Pilotte L, & Van den Eynde B.J. (2021). TGFβ1 neutralization displays therapeutic efficacy through both an immunomodulatory and a non-immune tumor-intrinsic mechanism. Journal for Immunotherapy of Cancer, 9(2), e001798.

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