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Alexa fluor 594 conjugated goat anti mouse igg1

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Alexa Fluor 594-conjugated goat anti-mouse IgG1 is a fluorescently-labeled secondary antibody. It is designed to detect and bind to mouse IgG1 primary antibodies.

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Lab products found in correlation

Alexa fluor 488 conjugated goat anti mouse igm, by Thermo Fisher Scientific (5 mentions) Alexa fluor 488 conjugated goat anti rabbit igg, by Thermo Fisher Scientific (4 mentions) Alexa fluor 488 conjugated α bungarotoxin, by Thermo Fisher Scientific (3 mentions) Alexa fluor 405 conjugated goat anti mouse igg2b, by Thermo Fisher Scientific (3 mentions) Laminin, by Merck Group (2 mentions) Alexa fluor 594 conjugated goat anti mouse igg, by Thermo Fisher Scientific (2 mentions) Alexa fluor 647 conjugated goat anti rabbit, by Thermo Fisher Scientific (2 mentions) Alexa fluor 647 conjugated goat anti rat igg, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488 conjugated goat anti mouse igg, by Thermo Fisher Scientific (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Bromodeoxyuridine (brdu), by Abcam (1 mentions) Alexa fluor 488 conjugated goat anti rat igg, by Thermo Fisher Scientific (1 mentions) Mouse anti human cd31, by Agilent Technologies (1 mentions) Alexa fluor 488 conjugated donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Nb300 130, by Novus Biologicals (1 mentions) Target retrieval solution ph 9, by Agilent Technologies (1 mentions) Bml pw9860, by Enzo Life Sciences (1 mentions) Eclipse ti microscope, by Nikon (1 mentions) Alexa fluor 647 conjugated α bungarotoxin, by Thermo Fisher Scientific (1 mentions) Mouse anti pax7, by Developmental Studies Hybridoma Bank (1 mentions)

Spelling variants of this product

Alexa Fluor 594 goat anti-mouse (177 citations) Alexa Fluor 594 anti-mouse (84 citations) Goat anti-mouse Alexa 594 (49 citations) Goat anti-mouse IgG1 (22 citations) Alexa Fluor 594-conjugated goat anti-mouse (20 citations) Alexa Fluor 594-conjugated anti-mouse (18 citations) Alexa Fluor 594 goat anti-mouse IgG1 (12 citations) Anti-mouse IgG1 Alexa-Fluor 594 (4 citations) Alexa Fluor 594–conjugated anti‐goat (2 citations) Goat anti-mouse IgG1 594 (2 citations)

8 protocols using alexa fluor 594 conjugated goat anti mouse igg1

1

Immunolabeling of Neuromuscular Junctions

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Pax7 (mouse IgG1, 1:100, Developmental Studies Hybridoma Bank (DSHB), Iowa City, IA), BrdU (rat, 1:250, Abcam ab6326, Cambridge, UK), Laminin (rat or rabbit, 1:1500, Sigma–Aldrich L0663 or L9393), GFP (rabbit, 1:400, Millipore AB3030P, Billerica, MA), F1.652 (mouse IgG1, 1:40, DSHB), A4.840 (mouse IgM, 1:40, DSHB), NCL-MHC (mouse IgG1, 1:100, DSHB), SC-71 (mouse IgG1, 1:40, DSHB), 6H1 (mouse IgM, 1:40, DSHB), BF-35 (mouse IgG1, 1:40, DSHB), BF-F3 (mouse IgG1, 1:40, DSHB), SV2 (synaptic vesicle protein-2, mouse IgG1, 1:100, DSHB), Znp-1 (synaptotagmin-2, mouse IgG1, 1:200, DSHB) and 2H3 (neurofilament, mouse IgG1, 1:200, DSHB), AlexaFluor 488-conjugated α-Bungarotoxin (1:1000, Life Technologies B-13422, Grand Island, NY), AlexaFluor 647-conjugated α-Bungarotoxin (1:1000, Life Technologies B-35450), AlexaFluor 594-conjugated goat anti-mouse IgG (1:1500, Life Technologies A-11032), AlexaFluor 594-conjugated goat anti-mouse IgG1 (1:1500, Life Technologies A-21125), AlexaFluor 488-conjugated goat anti-mouse IgM (1:1500, Life Technologies A-21042), AlexaFluor 488-conjugated goat anti-rat IgG (1:1500, Life Technologies A-11006), AlexaFluor 647-conjugated goat anti-rabbit (1:1500, Life Technologies A-21244).
Liu W., Wei-LaPierre L., Klose A., Dirksen R.T, & Chakkalakal J.V. (2015). Inducible depletion of adult skeletal muscle stem cells impairs the regeneration of neuromuscular junctions. eLife, 4, e09221.
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2

Identification of HUVECs in Tissue Aggregates

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CD31 staining was used to identify the presence and spatial distribution of HUVECs located in and/or around the aggregates. The sample sections were incubated for 30 minutes in 10% FBS (diluted in phosphate buffered saline (PBS)) to prevent non-specific background staining (blocking). The samples were then incubated for 1 hour in the primary antibody, mouse anti-human CD31 (Dako, UK), at a dilution of 1:20 in PBS. The samples were then incubated for 1 hour in the secondary antibody, alexa-fluor 594-conjugated goat anti-mouse IgG1 (Life Technologies, UK), diluted 1:200 in PBS. DAPI staining was carried out using a DAPI-conjugated mounting medium without further adjustment. The samples were then ready for imaging.
Deegan A.J., Hendrikson W.J., El Haj A.J., Rouwkema J, & Yang Y. (2019). Regulation of endothelial cell arrangements within hMSC – HUVEC co-cultured aggregates. Biomedical Journal, 42(3), 166-177.
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3

Comprehensive Muscle and Nerve Immunolabeling

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The following antibodies were used: Pax7 [mouse IgG1, 1:100, Developmental Studies Hybridoma Bank (DSHB)], laminin (rabbit, 1:1500, Sigma-Aldrich, L9393; rat, 1:1500, Sigma-Aldrich, L0663), GFP (rabbit, 1:400, Millipore AB3030P), BA-D5 (MyHC-I, mouse IgG2b, 1:40, DSHB), SC-71 (MyHC-IIA, mouse IgG1, 1:40, DSHB), BF-F3 (MyHC-IIB, mouse IgM, 1:40, DSHB), SV2 (synaptic vesicle protein-2, mouse IgG1, 1:100, DSHB), Znp-1 (synaptotagmin-2, mouse IgG2a, 1:200, DSHB), 2H3 (neurofilament, mouse IgG1, 1:200, DSHB), Alexa Fluor 488-conjugated α-bungarotoxin (1:1000, Thermo Fisher Scientific, B-13422), Alexa Fluor 405-conjugated goat anti-mouse IgG2b (1:1500, Thermo Fisher Scientific, A-21141), Alexa Fluor 488-conjugated goat anti-mouse IgM (1:1500, Thermo Fisher Scientific, A-21042), Alexa Fluor 594-conjugated goat anti-mouse IgG1 (1:1500, Thermo Fisher Scientific, A-21125), DAPI (1:3000), Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:1500, Thermo Fisher Scientific, A-32731).
Bachman J.F., Klose A., Liu W., Paris N.D., Blanc R.S., Schmalz M., Knapp E, & Chakkalakal J.V. (2018). Prepubertal skeletal muscle growth requires Pax7-expressing satellite cell-derived myonuclear contribution. Development (Cambridge, England), 145(20), dev167197.
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4

Immunofluorescence Characterization of Muscle Fibers

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The following antibodies were used: laminin‐α2 (rat, 1:1500, Sigma‐Aldrich, L0663), DAPI (1:3000), BA‐D5 (MyHC‐I, mouse IgG2b, 1:40, Developmental Studies Hybridoma Bank DSHB), SC‐71 (MyHC‐IIA, mouse IgG1, 1:40, DSHB), BF‐F3 (MyHC‐IIB, mouse IgM, 1:40, DSHB), collagen type IV (COL‐4, rabbit polyclonal, 1:500, Abcam ab6586), fibronectin‐1 (FN1, rabbit polyclonal, 1:500, Sigma ab2033), Alexa Fluor 405‐conjugated goat anti‐mouse IgG2b (1:1500, Thermo Fisher Scientific, A‐21141), Alexa Fluor 488‐conjugated goat anti‐mouse IgM (1:1500, Thermo Fisher Scientific, A‐21042), Alexa Fluor 594‐conjugated goat anti‐mouse IgG1 (1:1500, Thermo Fisher Scientific, A‐21125), AlexaFluor 488‐conjugated goat anti‐mouse IgG (1:1500, Life Technologies, A‐11001), AlexaFluor 488‐conjugated goat anti‐rabbit IgG (1:1500, Life Technologies, A‐11034), and AlexaFluor 647‐conjugated goat anti‐rat IgG (1:1500, Life Technologies, A‐21247).
Kallenbach J.G., Bachman J.F., Paris N.D., Blanc R.S., O'Connor T., Furati E., Williams J.P, & Chakkalakal J.V. (2022). Muscle‐specific functional deficits and lifelong fibrosis in response to paediatric radiotherapy and tumour elimination. Journal of Cachexia, Sarcopenia and Muscle, 13(1), 296-310.
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5

Localization of p62 and Ubiquitin in Tissue

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Three-dimensional localization of p62 and ubiquitin was analyzed by double immunofluorescence staining and followed by reconstruction as previously described with minor modifications20 (link). One-μm FFPE tissue sections were cut, dewaxed, rehydrated and pretreated with Target Retrieval Solution pH9 (#S2367, Dako) for 20 min at 97 °C.
For double labelling immunofluorescence the primary antibodies anti-p62 (#BML PW9860, Enzo, Life Sciences, NY, USA, diluted 1:250 for 60 min at RT) and anti-ubiquitin (#NB300-130, Novus, Littleton, CO, diluted 1:100 for 30 min at RT) were used. Secondary antibodies used were Alexa Fluor 488-conjugated donkey anti-rabbit IgG (#A21206, Thermo Fisher Scientific) and Alexa Fluor 594-conjugated goat anti-mouse IgG1 (#A21125, Thermo Fisher Scientific). Anti-p62 antibody was labelled with Alexa Fluor 488 and anti-ubiquitin antibody with Alexa Fluor 594. After 1:100 dilution they were incubated for 60 min at RT. DNA was stained with DAPI and image analysis was performed as described above.
Schwertheim S., Kälsch J., Jastrow H., Schaefer C.M., Theurer S., Ting S., Canbay A., Wedemeyer H., Schmid K.W, & Baba H.A. (2020). Characterization of two types of intranuclear hepatocellular inclusions in NAFLD. Scientific Reports, 10, 16533.
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6

Fluorescent Immunostaining of Brain Sections

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Thirty-micrometer thick brain coronal sections immunostained with anti-GFP and anti-OFP antibodies using a standard procedure for free-floating immunohistochemistry. Primary antibodies used for staining were mouse IgG1 monoclonal anti-mCherry (cross reacts with OFP) (Clonetech, 632543) and chicken polyclonal anti-GFP (Abcam, ab13970). These were visualized using Alexa Fluor 594 conjugated goat anti-mouse IgG1 and Alexa Fluor 488-conjugated goat anti-chicken IgY secondary antibodies (Thermo Fisher Scientific), respectively. Sections were mounted onto slides and coverslipped using Immu-Mount and images with a Nikon Eclipse Ti microscope (Nikon, CA) using a ×20 objective.
Pothayee N., Maric D., Sharer K., Tao-Cheng J.H., Calac A., Bouraoud N., Pickel J., Dodd S, & Koretsky A. (2018). Neural precursor cells form integrated brain-like tissue when implanted into rat cerebrospinal fluid. Communications Biology, 1, 114.
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7

Immunohistochemical Analysis of Muscle Fiber Types

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Pax7 (mouse IgG1, 1:100, Developmental Studies Hybridoma Bank (DSHB), Iowa City, Iowa), Laminin (rabbit, 1:1500, Sigma-Aldrich L9393), GFP (rabbit, 1:400, Millipore AB3030P, Billerica, Massachusetts), SC-71 (MyHC-IIA, mouse IgG1, 1:40, DSHB), BF-F3 (MyHC-IIB, mouse IgG1, 1:40, DSHB), SV2 (mouse IgG1, 1:100, DSHB), Znp-1 (Syt-2, mouse IgG1, 1:200, DSHB) and 2H3 (neurofilament, mouse IgG1, 1:200, DSHB), AlexaFluor 488-conjugated α-Bungarotoxin (1:1000, Thermo Fisher Scientific B-13422), AlexaFluor 647-conjugated α-Bungarotoxin (1:1000, Thermo Fisher Scientific B-35450), AlexaFluor 594-conjugated goat anti-mouse IgG (1:1500, Thermo Fisher Scientific A-11032), AlexaFluor 594-conjugated goat anti-mouse IgG1 (1:1500, Thermo Fisher Scientific A-21125), AlexaFluor 488-conjugated goat anti-mouse IgM (1:1500, Thermo Fisher Scientific A-21042), AlexaFluor 488-conjugated goat anti-rabbit IgG (1:1500, Thermo Fisher Scientific A-32731), AlexaFluor 647-conjugated goat anti-rabbit (1:1500, Thermo Fisher Scientific A-21244).
Liu W., Klose A., Forman S., Paris N.D., Wei-LaPierre L., Cortés-Lopéz M., Tan A., Flaherty M., Miura P., Dirksen R.T, & Chakkalakal J.V. (2017). Loss of adult skeletal muscle stem cells drives age-related neuromuscular junction degeneration. eLife, 6, e26464.
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8

Immunofluorescence Staining of Muscle Markers

Cited 1 time
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The following antibodies were used as described in detail elsewhere30 (link),48 (link),52 (link),68 (link),81 : Mouse anti-MyoD (1:250, BD Biosciences #554130), rabbit anti-Myogenin (1:250, Abcam, ab124800), rat anti-Laminin (1:1000, Sigma-Aldrich, L0663), mouse anti-Pax7 (1:100, Developmental Studies Hybridoma Bank), BA-D5 (MyHC-I, mouse IgG2b, 1:40, DSHB), SC-71 (MyHC-IIA, mouse IgG1, 1:40, DSHB), BF-F3 (MyHC-IIB, mouse IgM, 1:40, DSHB), AlexaFluor 488-conjugated goat anti-mouse IgG (1:1500, Life Technologies, A-11001), AlexaFluor 488-conjugated goat anti-rabbit IgG (1:1500, Life Technologies, A-11034), AlexaFluor 647-conjugated goat anti-rat IgG (1:1500, Life Technologies, A-21247), Alexa Fluor 405-conjugated goat anti-mouse IgG2b (1:1500, Thermo Fisher Scientific, A-21141), Alexa Fluor 488-conjugated goat anti-mouse IgM (1:1500, Thermo Fisher Scientific, A-21042), Alexa Fluor 594-conjugated goat anti-mouse IgG1 (1:1500, Thermo Fisher Scientific, A-21125).
Paris N.D., Kallenbach J.G., Bachman J.F., Blanc R.S., Johnston C.J., Hernady E., Williams J.P, & Chakkalakal J.V. (2020). Chemoradiation impairs myofiber hypertrophic growth in a pediatric tumor model. Scientific Reports, 10, 19501.
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