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Fitc 11 utp

Manufactured by Roche
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FITC-11-UTP is a fluorescently labeled uridine triphosphate (UTP) molecule used in various molecular biology applications. The FITC (Fluorescein Isothiocyanate) label is attached to the UTP molecule, allowing for the detection and tracking of nucleic acid synthesis or other processes involving UTP incorporation.

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Lab products found in correlation

T7 or sp6 rna polymerase, by New England Biolabs (2 mentions) Dig 11 utp, by Roche (2 mentions) Pcrii topo vector, by Thermo Fisher Scientific (2 mentions) Dnase 1, by Promega (2 mentions)

2 protocols using fitc 11 utp

1

Designing and Validating ISH Probes

Check if the same lab product or an alternative is used in the 5 most similar protocols
ISH probes were designed to span 500–1000 base pairs and were targeted to CDS and/or UTR regions of each mRNA (see Table S2). Probes were designed to minimize cross-hybridization with off-target mRNAs, which was assessed using BLAST. For the detection of specific ORs, probes targeting multiple gene regions were typically generated and tested. Probe sequences were amplified by PCR using specific primers (Table S2), inserted into the pCRII-TOPO vector (ThermoFisher), and confirmed by restriction analysis and sequencing. DIG- and FITC-labeled antisense RNA probes were generated from 1 μg of linearized plasmid template using T7 or Sp6 RNA polymerases (NEB) and DIG-11-UTP (Roche) or FITC-11-UTP (Roche), treated with DNaseI (Promega), ethanol precipitated, and dissolved in a 30 μL volume of water.
van der Linden C.J., Gupta P., Bhuiya A.I., Riddick K.R., Hossain K, & Santoro S.W. (2020). Olfactory Stimulation Regulates the Birth of Neurons That Express Specific Odorant Receptors. Cell reports, 33(1), 108210.
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2

Designing and Validating ISH Probes

Check if the same lab product or an alternative is used in the 5 most similar protocols
ISH probes were designed to span 500–1000 base pairs and were targeted to CDS and/or UTR regions of each mRNA (see Table S2). Probes were designed to minimize cross-hybridization with off-target mRNAs, which was assessed using BLAST. For the detection of specific ORs, probes targeting multiple gene regions were typically generated and tested. Probe sequences were amplified by PCR using specific primers (Table S2), inserted into the pCRII-TOPO vector (ThermoFisher), and confirmed by restriction analysis and sequencing. DIG- and FITC-labeled antisense RNA probes were generated from 1 μg of linearized plasmid template using T7 or Sp6 RNA polymerases (NEB) and DIG-11-UTP (Roche) or FITC-11-UTP (Roche), treated with DNaseI (Promega), ethanol precipitated, and dissolved in a 30 μL volume of water.
van der Linden C.J., Gupta P., Bhuiya A.I., Riddick K.R., Hossain K, & Santoro S.W. (2020). Olfactory Stimulation Regulates the Birth of Neurons That Express Specific Odorant Receptors. Cell reports, 33(1), 108210.
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