Ril 6

Manufactured by R&D Systems

The RIL-6 is a laboratory equipment product offered by R&D Systems. It is a device used for performing immunoprecipitation and pull-down assays to study protein-protein interactions. The core function of the RIL-6 is to facilitate the isolation and purification of target proteins and associated complexes from cell or tissue lysates.

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Lab products found in correlation

4 protocols using ril 6

Monoclonal antibody depletion and checkpoint blockade in tumor model

Cited 2 times

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The monoclonal anti-CD8a (clone 53–6.72; BioXCell) was administered i.p. at 500 μg every 7 days from d-1 prior to tumor injection to maintain CD8 T cell depletion. Anti-PD-ligand 1 (PD-L1; clone 10F.9G2; BioXCell), or isotype IgG2b (clone LTF-2; BioXCell) were administered by 200 μg i.p. injections, as described previously (16 (link), 17 (link)) at d-1, d0, d1 and d3 with respect to vaccine boost. Mouse rIL-6 (R&D Systems) suspended in PBS was given at 60 ng i.p. daily from d0 to d3 at boost (18 (link)).

Badamchi-Zadeh A., Moynihan K.D., Larocca R.A., Aid M., Provine N.M., Iampietro M.J., Kinnear E., Penaloza-MacMaster P., Abbink P., Blass E., Tregoning J.S., Irvine D.J, & Barouch D.H. (2018). Combined HDAC and BET Inhibition Enhances Melanoma Vaccine Immunogenicity and Efficacy. The Journal of Immunology Author Choice, 201(9), 2744-2752.

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CD4+ T Cell Activation Dynamics

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CD4⁺ T cells were purified from spleens of uninfected BALB/c CD45.1⁺ (CByJ.SJL(B6)-Ptprc^a/J), BALB/c and Chi3l1^-/- mice using CD4 MACS L3T4 beads (Miltenyi Biotech) and stimulated for 48 hours as previously described (41 (link)) with 2.5 μg/mL plate-bound anti-CD3 (145-2C11, BioXcell) and 2.5μg/ml plate-bound anti-CD28 (37.51, eBioscience). To mimic early T_FH development cells were cultured in the presence of 10 μg/ml rIL-6 (R&D) and neutralizing antibodies to IL-2 (JES6-1A12, BioXcell, 5 μg/ml and S4B6-1 BioXcell, 5 μg/mL). For CD4 T cell co-cultures, BALB/c or Chi3l^-/- CD45.2⁺ T cells were mixed at a 1:1 ratio with CD45.1⁺ T cells and then stimulated for 48 hours with CD3 and CD28 antibodies.

Curtiss M.L., Rosenberg A.F., Scharer C.D., Mousseau B., Benavides N.A., Bradley J.E., León B., Steele C., Randall T.D, & Lund F.E. (2023). Chitinase-3-like 1 regulates TH2 cells, TFH cells and IgE responses to helminth infection. Frontiers in Immunology, 14, 1158493.

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Activation of Naïve CD4+ T Cells

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Naïve CD4+ cells were purified from spleens of B6 mice using positive selection with anti-CD4 MACS beads (Miltenyi Biotec). Purified cells were activated with anti-CD3 (clone 145–2C11, 2.5μg/mL) and anti-CD28 (clone 37.51, 2.5μg/mL) antibodies in the presence of the indicated concentration of anti-IL-2 neutralizing antibodies (JES6–1A12 and S4B6–1, BioXcell) with or without rIL6 (R&D) at indicated concentrations. Cells were cultured for 48 h at 37 °C in 125 μl in round- bottomed 96-well plates in RPMI-1640 supplemented with sodium pyruvate, HEPES (pH 7.2–7.6 range), nonessential amino acids, penicillin, streptomycin, 2-mercaptoethanol and 10% heat-inactivated FCS (all from Gibco).

Papillion A., Powell M.D., Chisolm D.A., Bachus H., Fuller M.J., Weinmann A.S., Villarino A., O’Shea J.J., León B., Oestreich K.J, & Ballesteros-Tato A. (2019). Inhibition of IL-2 responsiveness by IL-6 is required for the generation of GC-TFH cells.. Science immunology, 4(39), eaaw7636.

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ATDC5 Chondrocyte Maturation and IL-6 Response

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ATDC5 cells (Sigma, 99072806) were maintained in DMEM/F-12 (1:1) medium (Gibco, 11330032) supplemented with 5% FBS and 1% penicillin/streptomycin. ATDC5 cells were maturated in DMEM/F-12 (1:1) medium supplemented with 5% FBS, 1% penicillin/streptomycin, 1% ITS premix (Corning, 354352), 50μg/ml ascorbic acid, 10nM dexamethasone, and 10ng/ml TGF-β3 (Sigma, SRP6552) for 5, 10, and 15 days.
Both wild type and Adgrg6 KO ATDC5 cells were treated with 100ng/ml recombinant human IL-6 protein (rIL-6) (R&D System, 206-IL) for 2 hours before protein extraction. Both wild type and Adgrg6 KO ATDC5 cells were treated with 10nM Stattic in maturation medium for 10 days before RNA extraction.

Liu Z., Easson G.W., Zhao J., Makki N., Ahituv N., Hilton M.J., Tang S.Y, & Gray R.S. (2019). Dysregulation of STAT3 signaling is associated with endplate-oriented herniations of the intervertebral disc in Adgrg6 mutant mice. PLoS Genetics, 15(10), e1008096.

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