The cervical cancer tissue microarrays, purchased from Shanghai Outdo Biotech (Shanghai, China), contained 85 cases of cancerous tissues with follow-up and 22 matiched cases of paracancerous tissues. This process had fully informed consent of the patients. Immunohistochemistry was performed by using the avidin–biotin complex method (Vector Laboratories), including heat-induced antigenretrieval procedures. Incubation with antibodies against CK5 (1:150; #71536, Cell Signaling), TIMP1 (1:150; abs149999, Absin), EZH2 (1:100; #5246, Cell Signaling), SF3B2 (1:150; abs116920, Absin), COPS8 (1:150; abs143431, Absin) was carried out at 4 °C for 12 h. PanCK CK5 was used to mark cancer cells. DAPI was used to highlight all nuclei. Visualization and quantitation of the different fluorophoreswas achieved on the TissueFAXS Spectra Systems and StrataQuest analysis software (TissueGnostics); the location information and expression of all the markers were computed for every cell were recorded.
Cervical cancer tissue microarray
Manufactured by Shanghai Outdo Biotech Co.
Sourced in China
The Cervical Cancer Tissue Microarray is a laboratory tool used for the analysis of cervical cancer tissue samples. It provides a standardized and efficient way to simultaneously examine multiple tissue samples on a single slide.
Automatically generated - may contain errors
7 protocols using cervical cancer tissue microarray
1
Cervical Cancer Tissue Microarray Analysis
2
Chemoradiotherapy for Cervical Cancer
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This study was approved by Ethics Committee of The Second Affiliated Hospital of Dalian Medical University. Fifty three patients diagnosed with cervical squamous cell carcinoma were recruited. Before the end of the statistics, all the patients did not undergo surgical resection, and all the patients received standard concurrent chemoradiotherapy. All patients signed informed consents before being enrolled into research and we comply with the Declaration of Helsinki when conducting the study. Cervical cancer tissue microarrays containing 119 patients were purchased from OutdoBiotech (Shanghai, China). The tumor tissue samples (tumor and adjacent tissue) were took from patients who had not undergone anti-tumor therapy since diagnosis, with all of the information frompatients authenticated.
3
Immunohistochemical Analysis of NEK2 in Cervical Cancer
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A cervical cancer tissue microarray, which contained 41 cervical carcinoma tissues and paired paracarcinoma tissues, was obtained from Shanghai Outdo Biotech (Shanghai, China). IHC analysis was performed as previously described [32 (link)–34 (link)]. Briefly, the tissue sections were deparaffinized, rehydrated, and blocked with goat serum. After incubation with the anti-NEK2 antibody (1:100, ab227958, Abcam) overnight at 4 °C, the sections were washed three times with PBS and incubated with an HRP-conjugated secondary antibody (1:300, K8002, Dako) for 30 min at room temperature. Following three 5-min rinses in PBS, the stained sections were reacted with 3,3′-diaminobenzidine for 10 min and then counterstained with 0.1% hematoxylin. The staining in tumor and normal tissues was scored, and the staining percentage was determined. The score calculated by multiplying the values assigned to the staining intensity and percentage was used to evaluate the expression of NEK2.
4
Clinicopathological Analysis of Cervical Cancer Samples
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Human cervical cancer tumours and adjacent non-tumour tissues were obtained from Guangxi Medical University (China). The clinicopathological characteristics of the samples are summarised in Table 1 . A cervical cancer tissue microarray was purchased from Shanghai Outdo Biotech Co. Ltd. (China). All patients provided informed consent for the use of their tissues before surgery. The study was approved by the Ethics Committee of the National Research Institute for Family Planning.
Statistical analysis of clinical samples
| Clincal samples pathological characteristics | n | CHN1 | CHN1 upregulated | Pearson Chi-Square | |||
|---|---|---|---|---|---|---|---|
| – | + | ++ | +++ | ||||
| 0.660 | 1.597 | ||||||
| ≤ 3 | 18 | 2 | 9 | 4 | 3 | ||
| > 3 | 28 | 2 | 12 | 11 | 3 | ||
| 0.269 | 7.594 | ||||||
| I | 2 | 1 | 1 | 0 | 0 | ||
| II | 38 | 2 | 18 | 12 | 6 | ||
| III | 6 | 1 | 2 | 3 | 0 | ||
| 0.962 | 0.290 | ||||||
| ≤ 1/2 muscular layer | 16 | 1 | 8 | 5 | 2 | ||
| >1/2 muscular layer | 30 | 3 | 13 | 10 | 4 | ||
| 0.008 | 11.895 | ||||||
| Absent | 23 | 2 | 15 | 4 | 2 | ||
| Present | 25 | 0 | 7 | 7 | 11 | ||
- (0 ≤ HSCORE < 75); + (75 ≤ HSCORE < 150); ++(151 ≤ HSCORE < 225); +++(225 ≤ HSCORE ≤300)
5
Cervical Cancer Tissue Microarray Analysis
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The cervical cancer tissue microarray was bought from Shanghai Outdo Biotech Co., Ltd. The tissue microarray was consisted of 31 cervical squamous cell carcinoma (mean age 41.0±2.6) tissues and paired adjacent non-tumor tissues. Patients meeting the following criteria were included: (1) all patient samples in this tissue microarray were pathologically diagnosed as cervical squamous cell carcinoma; (2) all patient samples required no additional treatment. And the exclusion criteria were as follows: patients with a history of chemotherapy or radiotherapy. The detailed clinic parameters of the enrolled patients were illustrated in Table 1 . The present study was approved by the Research Ethics Committee of Chao Yang Hospital of Capital Medical University. All patients provided informed consent for use of their samples.
6
Cervical Cancer Tissue Sampling and Cell Culture
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Human cervical cancer tumours and adjacent non-tumour tissues were obtained from Guangxi Medical University (China). The clinicopathological characteristics of the samples are summarised in Table 1. A cervical cancer tissue microarray was purchased from Shanghai Outdo Biotech Co. Ltd. (China). All patients provided informed consent for the use of their tissues before surgery. The study was approved by the Ethics Committee of the National Research Institute for Family Planning.
The human cervical carcinoma cell lines HeLa, SiHa, and C33A were purchased from the Cell Resource Center of Peking Union Medical College (Beijing, China) and cultured in Dulbecco's modified Eagle medium (DMEM) containing 10% foetal bovine serum (FBS), 100 IU/mL penicillin, and 10 mg/mL streptomycin. All cells were maintained at 37°C in an atmosphere containing 5% CO 2 .
The human cervical carcinoma cell lines HeLa, SiHa, and C33A were purchased from the Cell Resource Center of Peking Union Medical College (Beijing, China) and cultured in Dulbecco's modified Eagle medium (DMEM) containing 10% foetal bovine serum (FBS), 100 IU/mL penicillin, and 10 mg/mL streptomycin. All cells were maintained at 37°C in an atmosphere containing 5% CO 2 .
7
Cervical Cancer Tissue Sampling and Cell Culture
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Human cervical cancer tumours and adjacent non-tumour tissues were obtained from Guangxi Medical University (China). The clinicopathological characteristics of the samples are summarised in Table 1. A cervical cancer tissue microarray was purchased from Shanghai Outdo Biotech Co. Ltd. (China). All patients provided informed consent for the use of their tissues before surgery. The study was approved by the Ethics Committee of the National Research Institute for Family Planning.
The human cervical carcinoma cell lines HeLa, SiHa, and C33A were purchased from the Cell Resource Center of Peking Union Medical College (Beijing, China) and cultured in Dulbecco's modified Eagle medium (DMEM) containing 10% foetal bovine serum (FBS), 100 IU/mL penicillin, and 10 mg/mL streptomycin. All cells were maintained at 37°C in an atmosphere containing 5% CO 2 .
The human cervical carcinoma cell lines HeLa, SiHa, and C33A were purchased from the Cell Resource Center of Peking Union Medical College (Beijing, China) and cultured in Dulbecco's modified Eagle medium (DMEM) containing 10% foetal bovine serum (FBS), 100 IU/mL penicillin, and 10 mg/mL streptomycin. All cells were maintained at 37°C in an atmosphere containing 5% CO 2 .
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