Western blotting and immunohistochemical (fluorescence) staining were performed as described previously [8 (link),43 (link)]. The primary antibodies used in this study were actin monoclonal antibodies (1:5000 dilution; Sigma–Aldrich, A1978), FITC-conjugated anti-rabbit IgG, and rhodamine-conjugated anti-mouse IgG, alkaline phosphatase-conjugated anti-rabbit IgG antibody (1:500 dilution; Jackson ImmunoResearch Laboratories, 111–095-003, 115–025-146, 111–055-003), AHR (aryl hydrocarbon receptor) goat polyclonal antibody (1:200 dilution; Santa Cruz Biotechnology, sc-8088), ERN1, EIF2AK3, ATF6, EIF2A, p-EIF2A, ATG4, ATG16L1, ATG12, PIK3C3, LC3B and p-EIF2AK3 rabbit polyclonal antibody (1:500 dilution; Cell Signaling Technology, 3294, 5683, 65,880, 5324, 5199, 7613, 8089, 4180, 4263, 12,741, 3191, respectively). WLS and HSPA5 rabbit polyclonal antibody (1:500 dilution; Abcam, ab72385 and ab21685). All of the experiments were repeated at least three times.
Sc 8088
Manufactured by Santa Cruz Biotechnology
The Sc-8088 is a laboratory equipment designed for general use in research and analytical applications. It serves as a multi-purpose tool for various tasks within a controlled laboratory environment. The core function of the Sc-8088 is to provide a reliable and versatile platform for conducting scientific experiments and analyses. Further details about its intended use or specific applications are not available.
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Lab products found in correlation
Fitc conjugated anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
Ab21685, by Abcam (1 mentions)
Rhodamine conjugated anti mouse igg, by Jackson ImmunoResearch (1 mentions)
Alkaline phosphatase conjugated anti rabbit igg antibody, by Jackson ImmunoResearch (1 mentions)
Alexa fluor 594 conjugated donkey anti goat igg, by Thermo Fisher Scientific (1 mentions)
Lsm780 confocal laser scanning microscope, by Zeiss (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
Triton x 100, by Beyotime (1 mentions)
Antifade mounting medium, by Beyotime (1 mentions)
Paraformaldehyde (pfa), by Beyotime (1 mentions)
2 protocols using sc 8088
1
Comprehensive Protein Analysis by Western Blot and IHC
2
Immunofluorescence Staining of AhR
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The cells on coverslips (Nest) were fixed with 4% paraformaldehyde (Beyotime) in PBS for 30 min at room temperature, permeabilized with 0.5% Triton X-100 (Beyotime) in PBS for 10 min at room temperature, and then blocked with PBS containing 1% BSA and 1% goat serum for 30 min. The cells on coverslips were incubated with primary antibody to AhR (1:100, sc-8088, Santa Cruz) at 4°C overnight, followed by Alexa Fluor 594-conjugated donkey anti-goat IgG (1:200, Invitrogen) in the dark for 1 h in PBS containing 1% BSA and 0.5% Triton X-100, followed by DAPI (Beyotime) for 10 min. All incubations were performed followed by three washes with PBS. Coverslips were mounted with antifade mounting medium (Beyotime), placed on glass slides, and imaged using a LSM 780 laser-scanning confocal microscope (Carl Zeiss).
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