Experiments were carried out as described previously [15 (link)] and in the Extended Materials and Methods . Samples were prepared from microdissections of the dorsal raphe taken from ~1-month-old Cbln2 KO mice and control littermates. Intensity values for each protein of interest were first normalized to β-actin and then to the values in the control samples. Samples were collected from 6 mice/group. The following antibodies were used in this study: monoclonal mouse anti-β-actin (1:5000; A1978 Sigma-Aldrich; RRID: AB_476692), polyclonal rabbit anti-GluA1 (1:500; PC246 Millipore; RRID: AB_564636), monoclonal mouse anti-GluA2 (1:1000; MAB397 Millipore; RRID: AB_2113875), polyclonal rabbit anti-GluA4 (1:1000; AB1508 Millipore; RRID: AB_90711), monoclonal mouse anti-GluN1 (1:500; 114011 Synaptic Systems; RRID: AB_887750), monoclonal mouse anti-GluN2A (1:500; 75-288 Neuromab; RRID: AB_2315842), monoclonal mouse anti-GluN2B (1:500; 75-097 Neuromab; RRID: AB_10673405), polyclonal rabbit anti-HTR1A (1:1000; PA5-99483 Thermo Fisher Scientific; RRID: AB_2818416), and polyclonal rabbit anti-TPH2 (1:1000; NB100-74555 Novus; RRID: AB_2202792).
Nb100 74555 novus
Manufactured by Novus Biologicals
NB100-74555 Novus is a laboratory equipment product. It is designed for use in scientific research and analysis. The core function of this product is to provide a tool for researchers to conduct their experiments and studies.
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2 protocols using nb100 74555 novus
1
Quantification of Synaptic Proteins in Cbln2 KO Mice
2
Quantification of Synaptic Proteins in Cbln2 KO Mice
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Experiments were carried out as described previously [15 (link)] and in the Extended Materials and Methods . Samples were prepared from microdissections of the dorsal raphe taken from ~1-month-old Cbln2 KO mice and control littermates. Intensity values for each protein of interest were first normalized to β-actin and then to the values in the control samples. Samples were collected from 6 mice/group. The following antibodies were used in this study: monoclonal mouse anti-β-actin (1:5000; A1978 Sigma-Aldrich; RRID: AB_476692), polyclonal rabbit anti-GluA1 (1:500; PC246 Millipore; RRID: AB_564636), monoclonal mouse anti-GluA2 (1:1000; MAB397 Millipore; RRID: AB_2113875), polyclonal rabbit anti-GluA4 (1:1000; AB1508 Millipore; RRID: AB_90711), monoclonal mouse anti-GluN1 (1:500; 114011 Synaptic Systems; RRID: AB_887750), monoclonal mouse anti-GluN2A (1:500; 75-288 Neuromab; RRID: AB_2315842), monoclonal mouse anti-GluN2B (1:500; 75-097 Neuromab; RRID: AB_10673405), polyclonal rabbit anti-HTR1A (1:1000; PA5-99483 Thermo Fisher Scientific; RRID: AB_2818416), and polyclonal rabbit anti-TPH2 (1:1000; NB100-74555 Novus; RRID: AB_2202792).
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