Chi812b potentiostat

Amperometric measurements were performed with a CHI812B potentiostat (CH Instruments, Austin, TX, USA) controlled by software CHI812B. Screen-printed carbon electrodes (SPCEs) (DRP-110, DropSens, Llanera (Asturias), Spain), consisting of a 4-mm diameter carbon working electrode, a carbon counter electrode and an Ag pseudo-reference electrode, were used as electrochemical transducers together with a specific cable connector (DRP-CAC, DropSens, S.L.), which acted as interface between the SPCEs and the potentiostat.
A Bunsen AGT-9 Vortex (Velp Scientifica, Usmate, MB, Italy) was employed for homogenization of the solutions. A Thermomixer MT100 constant temperature incubator shaker (Universal Labortechnik, Leipzig, Germany), a magnetic separator DynaMag-2 Magnet (ThermoFisher Scientific,Waltham, MA, USA)) and a Magellan V 7.1 (TECAN, Männedorf, Switzerland) ELISA plate reader were also employed. Once MBs were conveniently modified, they were captured onto the surface of the SPCEs and controlled by a neodymium magnet (AIMAN GZ) embedded in a homemade Teflon casing. All measurements were performed at room temperature.

A CHI812B potentiostat (CH Instruments, Inc., Austin, TX, USA) operated by the software CHI812B was used for the electrochemical measurements. Screen-printed carbon electrodes (SPCEs, DRP-110) and the mandatory cable connector (DRP-CAC) were purchased from Methrom Hispania, S.I., (Madrid, Spain). A magnetic concentrator (DynaMag™-2, 123.21D, Invitrogen Dynal AS, Carlsbad, CA, USA) was used to efficiently separate and handle MBs. Other instruments employed include: a constant temperature incubator shaker (Optic Ivymen^® System, Comecta S.A, Scharlab, Madrid, Spain), a magnetic stirrer (Inbea S.L.), a Vortex Bunsen AGT-9, a Basic pH-meter (Basic 20+, Crison, Barcelona, Spain), and a homemade polymethacrylate (PMMA) casing with a neodymium magnet (AIMAN GZ) embedded. ELISA flat-bottom plates and a Magellan V 7.1 (TECAN) ELISA plate reader were used to compare the results obtained with the developed electrochemical immune platform.

Amperometric measurements were performed with a CHI812B potentiostat (CH Instruments, Austin, TX, USA) controlled by software CHI812B. SPCEs (DRP-110, DropSens, Llanera (Asturias), Spain), consisting of a 4-mm diameter carbon working electrode, a carbon counter electrode, and a Ag pseudo-reference electrode, were employed as transducers and a specific cable connector (DRP-CAC also from DropSens, S.L.) acted as an interface between the SPCEs and the potentiostat. All measurements were carried out at room temperature.
A Vortex Bunsen AGT-9 (Velp Scientifica, Usmate, MB, Italy) for the homogenization of the solutions, a Thermomixer MT100 constant temperature incubator shaker (Universal Labortechnik, Leipzig, Germany), a magnetic separator DynaMag™-2 Magnet (ThermoFisher Scientific, Waltham, MA, USA) and a Magellan V 7.1 (TECAN, Männedorf, Switzerland) ELISA plate reader were also employed. Capture of the modified MBs onto the SPCE surface was controlled by placing it in a homemade Teflon casing with an embedded neodymium magnet (AIMAN GZ) [15 (link)].