Fitc mouse igg1 κ isotype ctrl antibody

Cells were washed with PBS, detached using Trypsin/EDTA (PAN Biotech, Adenbach, Germany) and washed twice with PBS containing 2% BSA. Cells were stained with 5 µl FITC anti-human CD324 (E-cadherin) antibody (RRID : AB_756066, Biolegend, San Diego, CA, USA) or 5 µl FITC Mouse IgG1 κ Isotype Ctrl antibody (RRID : AB_2861401, Biolegend) for 30 min at 4°C in the dark. After two further washing steps, cells were measured with a Gallios flow cytometer (Beckman Coulter, Brea, CA, USA) and data were analyzed using Kaluza Analysis 2.1 software.

Passage 3 rat MSCs were digested by 0.25% trypsin, washed twice with PBS, and then resuspended in PBS as single cell suspension. The cells were incubated with fluorescence-coupled antibodies and isotype control antibodies, respectively (0.4 μg antibody: 1 × 10⁵ cells). The antibodies involved are as follows: PE anti-mouse/rat CD29 antibody (BioLegend, USA, catalog number: 102207), FITC anti-rat CD90 antibody (BioLegend, USA, catalog number: 206105), FITC mouse anti-rat CD44H (BD Biosciences, USA, catalog number: 550974), CD34 antibody-FITC (Santa Cruz Biotechnology, USA, catalog number: sc-7324 FITC), CD45 antibody-FITC (Santa Cruz Biotechnology, USA, catalog number: sc-1178 FITC), PE Armenian hamster IgG isotype Ctrl antibody (BioLegend, USA, catalog number: 400907), and FITC mouse IgG1, κ isotype Ctrl antibody (BioLegend, USA, catalog number: 400107). After 30 min of incubation at 4°C in the dark, rat MSCs were washed twice with PBS and resuspended in 300 μl PBS for analysis. Cell fluorescence was examined by a FACScan flow cytometer running CellQuest software.