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Mouse intesticult culture medium

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Mouse IntestiCult culture medium is a complete, serum-free medium designed for the maintenance and expansion of mouse intestinal organoids. The medium supports growth and differentiation of primary mouse intestinal cells in vitro.

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IntestiCult medium Mouse Intesticult Intesticult

2 protocols using mouse intesticult culture medium

1

Mouse Intestinal Organoid Generation

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Mouse organoids were generated from the same pool of mice used in mouse model experiments, with both sexes being used. Mouse tumors were dissociated using TrypLE Express, and cell pellets were resuspended in Matrigel and seeded in 25 μL droplets in a 24-well or 12-well plate. Once solidified, samples were incubated in 1 mL Mouse IntestiCult culture medium (STEMCELL Technologies) with 100 μg/mL Primocin for 5 days. Fresh media was replaced on day 3. Passaging was performed similarly to human organoids.
Chen B., Scurrah C.R., McKinley E.T., Simmons A.J., Ramirez-Solano M.A., Zhu X., Markham N.O., Heiser C.N., Vega P.N., Rolong A., Kim H., Sheng Q., Drewes J.L., Zhou Y., Southard-Smith A.N., Xu Y., Ro J., Jones A.L., Revetta F., Berry L.D., Niitsu H., Islam M., Pelka K., Hofree M., Chen J.H., Sarkizova S., Ng K., Giannakis M., Boland G.M., Aguirre A.J., Anderson A.C., Rozenblatt-Rosen O., Regev A., Hacohen N., Kawasaki K., Sato T., Goettel J.A., Grady W.M., Zheng W., Washington M.K., Cai Q., Sears C.L., Goldenring J.R., Franklin J.L., Su T., Huh W.J., Vandekar S., Roland J.T., Liu Q., Coffey R.J., Shrubsole M.J, & Lau K.S. (2021). Differential pre-malignant programs and microenvironment chart distinct paths to malignancy in human colorectal polyps. Cell, 184(26), 6262-6280.e26.
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2

Mouse Intestinal Organoid Generation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse organoids were generated from the same pool of mice used in mouse model experiments, with both sexes being used. Mouse tumors were dissociated using TrypLE Express, and cell pellets were resuspended in Matrigel and seeded in 25 μL droplets in a 24-well or 12-well plate. Once solidified, samples were incubated in 1 mL Mouse IntestiCult culture medium (STEMCELL Technologies) with 100 μg/mL Primocin for 5 days. Fresh media was replaced on day 3. Passaging was performed similarly to human organoids.
Chen B., Scurrah C.R., McKinley E.T., Simmons A.J., Ramirez-Solano M.A., Zhu X., Markham N.O., Heiser C.N., Vega P.N., Rolong A., Kim H., Sheng Q., Drewes J.L., Zhou Y., Southard-Smith A.N., Xu Y., Ro J., Jones A.L., Revetta F., Berry L.D., Niitsu H., Islam M., Pelka K., Hofree M., Chen J.H., Sarkizova S., Ng K., Giannakis M., Boland G.M., Aguirre A.J., Anderson A.C., Rozenblatt-Rosen O., Regev A., Hacohen N., Kawasaki K., Sato T., Goettel J.A., Grady W.M., Zheng W., Washington M.K., Cai Q., Sears C.L., Goldenring J.R., Franklin J.L., Su T., Huh W.J., Vandekar S., Roland J.T., Liu Q., Coffey R.J., Shrubsole M.J, & Lau K.S. (2021). Differential pre-malignant programs and microenvironment chart distinct paths to malignancy in human colorectal polyps. Cell, 184(26), 6262-6280.e26.
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