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Rat mpo elisa kit

Manufactured by USCN
Sourced in China

The Rat MPO ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of rat myeloperoxidase (MPO) levels in biological samples, such as serum, plasma, and cell culture supernatants. This kit uses a specific antibody coated on a 96-well plate to capture MPO, which is then detected by a second antibody conjugated with horseradish peroxidase (HRP). The HRP enzyme catalyzes a color-producing reaction, allowing the quantification of the target analyte.

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2 protocols using rat mpo elisa kit

1

Neutrophil Activation and Inflammation

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Anti-CaSR-Ab was purchased from NOVUS (Colorado, USA). Anti-P-65-Ab and anti-P-p65-Ab were from Cell Signaling Technology (Boston, USA). Histopaque1083, Histopaque1119, and fMLP were from Sigma (Darmstadt, Germany). NPS-2143, cincalcet, and PDTC were purchased from MCE (New Jersey, USA). Rat IL-6 ELISA kit was from Elabscience (Wuhan, China), rat MPO ELISA kit was from USCN (Wuhan, China), and rat IL-10 was from CUSABIO (Wuhan, China). DHE-ROS kit was from BestBio (Shanghai, China), and NO assay kit was from Beyotime (Shanghai, China).
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2

Quantifying Inflammatory Cytokines in Rat Astrocytes

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The expression levels of IL-1β and TNF-α in rat astrocytes were measured using Rat ELISA kit (Lianke, Hangzhou, China). The myeloperoxidase (MPO) production in astrocytes was accessed using Rat MPO ELISA kit (USCN, Wuhan, China). The optical density (OD) values at 450 nm and 570 nm were detected by ELX800 microplate reader (Biotek, Biotek Winooski, VT, USA).
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