All chemical reagents used in this work were of analytical grade and water was purified by a Milli-Q® Reference (Nippon Millipore, Tokyo, Japan) system. ENG hard resin used for fabrication of the on-chip injector and a template for microfluidic device was obtained from Kudo 3D Inc. (CA, U.S.A.). Polydimethylsiloxane (PDMS) prepolymer and curing agent were purchased from Dow Corning Toray Co., Ltd. (Tokyo, Japan). Isopropyl alcohol (IPA), hydrochloric acid, sodium dihydrogen phosphate, disodium hydrogen phosphate, sodium chloride, ethanol and acetone were obtained from Kanto Chemical Co., Inc. (Tokyo, Japan). Tris(hydroxymethyl)aminomethane (Tris) was obtained from MP Biomedicals, Inc. (CA, U.S.A.). Food dyes (yellow and blue) were purchased from Kyoritsu Foods Co., Ltd. (Tokyo, Japan). Resorufin sodium salt and bovine serum albumin (BSA) were obtained from Sigma-Aldrich Co. LLC (MO, U.S.A.). Amplex Red was obtained from Thermo Fisher Scientific, Inc. (MA, USA). Hydrogen peroxide and horseradish peroxidase (HRP) were purchased from FUJIFILM Wako Pure Chemical Corp. (Tokyo, Japan).
Sodium dihydrogen phosphate
Manufactured by Kanto Chemical
Sourced in Japan
Sodium dihydrogen phosphate is a chemical compound with the formula NaH2PO4. It is a salt that is commonly used as a buffer in various laboratory applications. Sodium dihydrogen phosphate is a white, crystalline powder that is soluble in water.
Automatically generated - may contain errors
Lab products found in correlation
Sodium chloride (nacl), by Kanto Chemical (2 mentions)
Disodium hydrogen phosphate, by Kanto Chemical (2 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Amplex red, by Thermo Fisher Scientific (1 mentions)
Hydrogen peroxide, by Fujifilm (1 mentions)
Resorufin sodium salt, by Merck Group (1 mentions)
Acetone, by Kanto Chemical (1 mentions)
Horseradish peroxidase hrp, by Fujifilm (1 mentions)
Ethanol, by Kanto Chemical (1 mentions)
Tris hydroxymethyl aminomethane tris, by MP Biomedicals (1 mentions)
Potassium chloride (kcl), by Kanto Chemical (1 mentions)
Sodium hydroxide (naoh), by Kanto Chemical (1 mentions)
Potassium dihydrogen phosphate, by Kanto Chemical (1 mentions)
Acetic acid, by Kanto Chemical (1 mentions)
Sodium acetate, by Kanto Chemical (1 mentions)
Milli q system, by Merck Group (1 mentions)
2 2 azobis isobutyronitrile, by Tokyo Chemical Industry (1 mentions)
Ethylene diamine, by Tokyo Chemical Industry (1 mentions)
Disodium hydrogen phosphate, by Nacalai Tesque (1 mentions)
Human hemoglobin hb, by Merck Group (1 mentions)
4 protocols using sodium dihydrogen phosphate
1
Fabrication and Application of Microfluidic Devices
2
Cellulase-Assisted Hydrogel Synthesis
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CMC (molecular weight = 8–10 × 104 as specified by manufacturer) with a 0.70 degree of substitution was obtained from Daicel Co., (Osaka, Japan). EGDE was purchased from Fujifilm Wako Pure Chemical Co., Ltd. (Osaka, Japan). Liquid paraffin (Moresco white P-100, 19.04 mm2/s) was purchased from Moresco Co., (Kobe, Japan). Sodium hydroxide was purchased from Kanto Chemical Co., Inc. (Tokyo, Japan). Methanol was purchased from Godo Co., Ltd. (Tokyo, Japan). The SPA was supplied by S. T. Co., (Tokyo, Japan). Trichoderma viride cellulase ONOZUKA R-10 was purchased from Yakult Pharmaceutical Co., Ltd. (Tokyo, Japan). The PBS and sodium acetate buffer used were analytical grade chemicals, i.e., sodium chloride, potassium chloride, sodium dihydrogen phosphate, potassium dihydrogen phosphate, sodium acetate, and acetic acid, purchased from Kanto Chemical Co., Inc. (Tokyo, Japan).
3
Affinity-Based Monoclonal Antibody Purification
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Sodium dihydrogen phosphate, 2-hydrate, disodium hydrogen phosphate, anhydrous (Kanto Chemical Co., Inc., Tokyo, Japan), and water (Milli-Q system; Merck Millipore, Darmstadt, Germany) were used in preparing the mobile phase, 100 mM phosphate buffer (pH 7.0). Tetraethoxysilane (TEOS), 3-aminopropyltrimethoxysilane (APTES), 3-methacryloxypropyltrimethoxysilane (MPS), and 3-glycidyloxypropyltrimethoxysilane (GPTMS) were obtained from Shin-Etsu Chemical (Tokyo, Japan). Polyethyehylene oxide, Immunoglobulin G human, and Protein A were provided by Sigma-Aldrich Co. (St. Louis, MO, USA).
N-Disuccinimidyl carbonate (DSC), glycidyl methacrylate (GMA), 2-hydroxyethyl methacrylate (HEMA), N,N-dimethylformamide (DMF), D-glucitol, ethylene diamine, and 2,2′-azobisisobutyronitrile (AIBN) were obtained from Tokyo Chemical Industry Co. (Tokyo, Japan). A Chinese hamster ovary (CHO) cell culture medium containing monoclonal antibody was obtained from manufacturing technology association of pharmaceutical that asked for confidentiality. All other reagents were of analytical grade and were purchased from KISHIDA Chemicals (Tokyo, Japan).
N-Disuccinimidyl carbonate (DSC), glycidyl methacrylate (GMA), 2-hydroxyethyl methacrylate (HEMA), N,N-dimethylformamide (DMF), D-glucitol, ethylene diamine, and 2,2′-azobisisobutyronitrile (AIBN) were obtained from Tokyo Chemical Industry Co. (Tokyo, Japan). A Chinese hamster ovary (CHO) cell culture medium containing monoclonal antibody was obtained from manufacturing technology association of pharmaceutical that asked for confidentiality. All other reagents were of analytical grade and were purchased from KISHIDA Chemicals (Tokyo, Japan).
4
Characterizing Hemoglobin-Sodium Dithionite Interactions
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Sodium dithionite (SDT) was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Human hemoglobin (Hb) was obtained from Sigma-Aldrich (St. Louis, MO) and was employed as a model of HBOCs. Disodium hydrogen phosphate was purchased from Nacalai tesque (Kyoto, Japan). Sodium dihydrogen phosphate was purchased from Kanto Chemical Co., Inc., (Tokyo, Japan). One hundred mM phosphate buffer (pH 7.4) was prepared with Disodium hydrogen phosphate and Sodium dihydrogen phosphate. Hb (0.83 mg/mL) and SDT (0.16 -10 mg/mL) solutions were prepared with 100 mM phosphate buffer (pH 7.4).
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