Alexa Fluor-conjugated secondary antibodies were obtained from Life Technologies (Carlsbad, CA), IRDye-conjugated anti-mouse and anti-rabbit secondary antibodies were obtained from LI-COR Biosciences (Lincoln, NE). Mouse anti-smMHC, anti-sm-α-actin, anti-calponin, rabbit anti-fibronectin were obtained from Sigma (St. Louis, MO) and anti-phospho Smad2 and anti-Smad2 antibodies were purchased from Cell Signaling (Danvers, MA). Sircol collagen assay was obtained from Biocolor Life Sciences, UK. Staining kits (PAS, Trichrome), chloroquine, quinine and saccharine were obtained from Sigma. H&E and Afog staining kits were obtained from Vector Labs. All other chemicals were of analytical grade.
Anti sm α actin
Manufactured by Merck Group
Sourced in Sao Tome and Principe, Japan
Anti-sm-α-actin is a laboratory reagent used for the detection and quantification of smooth muscle α-actin, a protein found in the contractile apparatus of smooth muscle cells. It is commonly used in immunohistochemistry and Western blotting applications to identify and analyze the presence and distribution of smooth muscle cells in various tissues and samples.
Automatically generated - may contain errors
Lab products found in correlation
Saccharine, by Merck Group (1 mentions)
Anti calponin, by Merck Group (1 mentions)
Rabbit anti fibronectin, by Merck Group (1 mentions)
Quinine, by Merck Group (1 mentions)
Irdye conjugated anti mouse and anti rabbit secondary antibodies, by LI COR (1 mentions)
Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)
N propyl gallate, by Merck Group (1 mentions)
Cyanine3 (cy3), by Thermo Fisher Scientific (1 mentions)
P75ntr, by Merck Group (1 mentions)
Alexa 488, by Thermo Fisher Scientific (1 mentions)
Anti α tubulin antibody, by Merck Group (1 mentions)
Alexa 568 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)
Anti flag m2 affinity gel, by Merck Group (1 mentions)
3 protocols using anti sm α actin
1
Immunohistochemical Analysis of Cellular Markers
2
Immunostaining of Olfactory Ensheathing Cells
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For phenotypic identification of OECs in uninfected and infected cultures, the fixed cultures were incubated with rabbit polyclonal anti-p75 neurotrophin receptor antibodies (p75 NTR –1/400, Sigma) and an anti-smooth muscle α-Actin (anti-SM α-Actin – 1/100, Sigma). After reaction with the primary antibodies of interest, cells were incubated with goat anti-rabbit IgG and sheep or goat anti-mouse IgG secondary antibodies, labeled with either Alexa 488 or Cy3 (Life Technologies; Invitrogen, Carlsbad, CA, USA), washed in pH 7.4 PBS, mounted with N-propylgallate (Sigma) in PBS–glycerol and coverslipped.
3
Antibodies and Plasmids Used in Myogenesis Study
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Antibodies used in this study were as follows: anti-DYKDDDDK (anti-Flag) antibody (Trans Genic, Kobe, Japan), anti-SMα−actin, anti-α−tubulin antibodies, and anti-Flag M2 affinity gel (Sigma, St. Louis, MO), anti-myoD and anti-myogenin antibodies (Santa Cruz Biotechnology, Santa Cruz, CA), anti-myosin heavy chain (MHC) antibody (MF20) (Developmental Studies Hybridoma Bank), and Alexa 568-conjugated secondary antibody (Molecular Probes, Eugene, OR). Concerning a polyclonal anti-MRTF-A antibody, we described in detail previously [13 (link)]. Detail information about the plasmids used in this study was described elsewhere [8 (link), 22 (link)].
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