Mouse monoclonal anti lamp1

HA was a kind gift from Prof. Ivan Donati, Department of Life Sciences, University of Trieste. The following antibodies were used: mouse monoclonal anti-vimentin, mouse monoclonal anti-LAMP1, mouse monoclonal anti-HAS2 (#sc-365263) and mouse monoclonal anti-actin purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA); mouse monoclonal anti-EEA1, mouse monoclonal anti-Rab11A, mouse monoclonal anti-calnexin, mouse monoclonal anti-TGN38, streptavidin Cy3-conjugated and anti-mouse IgG fluorescein isothiocyanate (FITC)-conjugated from Abcam (Abcam Inc, Toronto, ON, Canada); rabbit polyclonal anti-C1q from Dako (Dako, Glostrup, Denmark); rabbit polyclonal anti-HAS1 (#PA5-50674) from Invitrogen^TM (Thermo Fisher Scientific, Waltham, MA, USA); rabbit polyclonal anti-HAS3 (#15609-1-AP) from Proteintech (Proteintech, Rosemount, IL, USA); anti-mouse LI-COR IRDye 680RD and anti-rabbit LI-COR IRDye 800CW from LI-COR Biosciences (LI-COR Biosciences, Lincoln, NE, USA). All chemicals were purchased from Sigma (Sigma-Aldrich, Saint Louis, MO, USA).

The following antibodies were used: rabbit polyclonal anti-Bcl-xL (54H6) (Cell Signaling Technology, 2764), rabbit polyclonal anti-Atg14 (Sigma-Aldrich, A6358), mouse monoclonal anti-α-tubulin (Sigma-Aldrich, T6199), mouse monoclonal anti-FLAG M2 (Sigma-Aldrich, F1804), mouse monoclonal anti-GFP (GF200) (Nacalai Tesque, 04363–24), rabbit polyclonal anti-LC3B (Sigma-Aldrich, L7543), mouse monoclonal anti-LAMP1 (Santa Cruz, sc-20011), rabbit polyclonal anti-Beclin 1 (Cell Signaling Technology, 3738), rabbit polyclonal anti-Atg5 (D1G9) (Cell Signaling Technology, 8540), rabbit polyclonal anti-Atg7 (D12B11) (Cell Signaling Technology, 8558), and mouse monoclonal anti-Galectin-3 (BD Biosciences, 556904). The secondary antibodies used for immunoblotting were horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG (Jackson Immunoresearch Laboratories). The fluorescent secondary antibodies used for immunofluorescence were Alexa Fluor 488-conjugated goat anti-rabbit IgG (Molecular Probes/Invitrogen), Alexa Fluor 568-conjugated goat anti-mouse IgG (Molecular Probes/Invitrogen), or Alexa Fluor 594-conjugated goat anti-mouse IgG (Molecular Probes/Invitrogen).

The following antibodies were used: mouse monoclonal anti-FLAG M2 (Sigma-Aldrich), mouse monoclonal anti-ubiquitinated protein (FK2; Nippon Bio-Test Laboratories), mouse monoclonal anti-Rab30 (Abcam, ab156774), rabbit monoclonal anti-Atg5 (Cell Signaling Technology, #4967), rabbit monoclonal anti-β-actin (Cell Signaling Technology, #4970), mouse monoclonal anti-LAMP1 (Santa Cruz, sc-20011), and rabbit monoclonal anti-GM130 (BD Transduction Laboratories, 610822). The secondary antibodies used for immunoblotting were horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG (Jackson Immunoresearch Laboratories). The fluorescent secondary antibodies used for immunofluorescence were Alexa Fluor 488-conjugated goat anti-mouse or anti-rabbit IgG, Alexa Fluor 594-conjugated goat anti-mouse or anti-rabbit IgG, or Alexa Fluor 660-conjugated goat anti-mouse or anti-rabbit IgG (Molecular Probes/Invitrogen).