Human lymphocyte isolation medium

IL-6 and IL-17 ELISA kits were from eBioscience (San Diego, California, USA). Human lymphocyte isolation medium, recombinant human IL-2 (rIL-2), phytohemagglutinin (PHA), and 4-AP were purchased from Sigma–Aldrich (St. Louis, Missouri, USA). The MACS kit was from Miltenyi Biotec (Germany), and telmisartan was from Boehringer–Ingelheim (Germany). Fetal bovine serum (FBS) and Roswell Park Memorial Institute (RPMI) 1640 medium were obtained from HyClone (New Zealand). Fluorescein isothiocyanate (FITC)-labelled mouse anti-human CD3 and FITC-labelled mouse anti-human CD4 were from Becton, Dickinson and Company (USA). TRIzol was from Life Technologies (USA). The RT kit, Radio-Immunoprecipitation Assay (RIPA) lysis buffer, protease inhibitor, bicinchoninic acid (BCA) protein assay kit, and enhanced chemiluminescence (ECL) Western blotting kit were from Thermo Fisher (USA). The real-time fluorescent qPCR kit was from Qiagen (Germany). Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS–PAGE) reagents were purchased from Bio-Rad (USA). Anti-human monoclonal antibodies against the Kv1.3 potassium channel and β-actin and secondary antibodies were obtained from Abcam (UK). The 5-bromo-4-chloro-3’-indolyphosphate/nitro-blue tetrazolium chromogenic reagent was from Invitrogen (USA).

Reagent used were: human lymphocyte isolation medium (Sigma, USA); Pan T Cell Isolation Kit (Milteni, Germany); 10% foetal bovine serum (FBS) (Hyclone, New Zealand); RPMI 1640 medium (Hyclone, New Zealand); rIL-2 (Sigma, US); FITC-CD3 (Becton, Dickinson and Company, USA); Trizol (Life Technologies, USA); Reverse transcription kit (Thermo Fisher, USA); RT-PCR kit (Qiagen, Germany); RIPA Lysis Buffer (Thermo Fisher, USA); Protease inhibitor (Thermo, USA); BCA Protein Assay Kit (Thermo, USA); SDS-PAGE (BIO-RAD, USA); anti-human monoclonal antibodies against NFATc1, IL-6, TNF-α, and β-actin, and goat anti-rabbit secondary antibodies (Abcam, UK); BCIP/NBT chromogenic reagent (Invitrogen, USA); telmisartan (Boehringer-Ingelheim, Germany); CsA (Novartis, Switzerland); VIVIT (Sigma, USA); 4-AP (Sigma, USA).