The levels of triglyceride (TG), blood urea nitrogen (BUN), total billiubin (T-bill), glutamic-oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) in plasma were enzymatically measured using commercially available kits (ARKRAY, Inc., Minami-ku, Kyoto, Japan). Plasma total cholesterol, low density lipoprotein (LDL)-cholesterol and HDL-cholesterol were determined using HDL and LDL/very low density lipoprotein (VLDL) Assay kit (E2HL-100, BioAssay Systems, Hayward, CA, USA). The plasma concentration ofleptin and insulin were measured based on the ELISA method using commercial rat leptin and insulin ELISA kit (Leptin Rat ELISA ab100773, Abcam, Cambridge, MA, USA; Insulin, 80-INSRT-E01, ALPCO, Cambridge, MA, USA).
E2hl 100
Manufactured by BioAssay Systems
Sourced in United States
The E2HL-100 is a laboratory equipment product manufactured by BioAssay Systems. It is designed to perform specific tasks within a laboratory setting. The core function of the E2HL-100 is to [provide a concise, factual, and unbiased description of the product's core function without interpretation or extrapolation].
Automatically generated - may contain errors
Lab products found in correlation
Leptin rat elisa ab100773, by Abcam (1 mentions)
Ab100773, by Abcam (1 mentions)
80 insrt e01, by ALPCO (1 mentions)
Ealt 100, by BioAssay Systems (1 mentions)
Eastr 100, by BioAssay Systems (1 mentions)
Digl 100, by BioAssay Systems (1 mentions)
Ecch 100, by BioAssay Systems (1 mentions)
Mbs726298, by MyBioSource (1 mentions)
Etga 200, by BioAssay Systems (1 mentions)
Microvette cb 300 lh tubes, by Sarstedt (1 mentions)
5 protocols using e2hl 100
1
Plasma Biomarker Measurement Protocol
2
Plasma Lipid Profile Measurement
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The levels of triglyceride in plasma were measured by using commercial kits (ARKRAY, Inc., MINAMI-KU, KYOTO, Japan). The levels of high-density lipoprotein (HDL)-cholesterol, total cholesterol, and LDL-cholesterol in plasma were measured by using HDL and LDL assay kit (E2HL-100, BioAssay Systems).
3
Plasma Biomarker Measurement Protocol
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Triglyceride levels in plasma were measured using commercial kits (AM 157S-K, ASAN, Korea). Levels of HDL, total cholesterol, and low-density lipoprotein (LDL) in plasma were measured using HDL and LDL assay kits (E2HL-100, Bio Assay Systems, Germany). Levels of insulin in plasma were measured using commercial kits (80-INSRT-E01, ALPCO, UK). Levels of C-reactive protein (CRP) in plasma were measured using commercial kits (557825, BD Biosciences, America). Levels of leptin in plasma were measured using commercial kits (ab100773, Abcam, UK). Levels of T-Bill and BUN in plasma were measured using commercial kits (77184, Arkray, Japan).
4
Metabolic Biomarkers in Rat Models
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The fasting glucose and insulin levels were assessed using commercially available kits formulated specifically for rats (#90010, Crystal Chem, Elk Grove Village, IL, USA and #DIGL-100, BioAssay Systems, Hayward, CA, USA) following each supplier’s instructions. In addition, we calculated the values from the homeostatic model assessment of β-cell function (HOMA-β) for each animal using the previously published equation [30 (link)]: HOMA-β = (Fasting insulin (ng/mL) × 20)/(Fasting glucose (mg/dL) − 3.5). Levels of total cholesterol (CHOL) and triglyceride (TG) serum levels were determined using multi-enzyme-based kits (#MBS726298, MyBioSource, San Diego, CA, USA, and #ECCH-100, BioAssay Systems, Hayward, CA, USA, respectively). Serum and hepatic levels of high-/low-density lipoprotein cholesterol (LDL-c/HDL-c were assayed using a special colorimetric kit (#E2HL-100 BioAssay Systems, CA, USA). Serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase were analyzed using assay kits (#EALT-100 and EASTR-100, BioAssay Systems, CA, USA). All procedures were performed according to the manufacturer’s instructions for each kit (n = 8 samples/group).
5
Plasma Lipid and Glucose Profiling
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After blood was drawn by cardiac puncture, plasma was isolated using Microvette CB 300 LH tubes (Sarstedt). The concentrations of total cholesterol, LDL/VLDL cholesterol, HDL cholesterol and triglycerides were determined using enzymatic colorimetric assays (#E2HL-100 and #ETGA-200; BioAssay Systems) as per the manufacturer's instructions. Glucose levels were detected using the Contour®Next EZ Blood Glucose Monitoring System with Contour®Next test strips.
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