Alcian blue pas stain kit

Manufactured by Abcam

Sourced in United Kingdom

The Alcian Blue PAS Stain Kit is a laboratory tool used to stain and differentiate between acidic and neutral mucins in tissue samples. It utilizes a combination of Alcian Blue and Periodic Acid-Schiff (PAS) staining techniques to provide a visually distinct identification of glycosaminoglycans and other carbohydrate-rich structures within the sample.

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Lab products found in correlation

Proteome profiler mouse xl cytokine array kit, by R&D Systems (1 mentions) Diaminobenzidine dab kit, by Vector Laboratories (1 mentions) Cas block, by Thermo Fisher Scientific (1 mentions) Claudin 2, by Abcam (1 mentions) Streptavidin hrp, by Thermo Fisher Scientific (1 mentions) Versa 8, by Leica (1 mentions) Toluidine blue, by Merck Group (1 mentions) Antibiotic antimycotic solution 100x, by Merck Group (1 mentions)

3 protocols using alcian blue pas stain kit

Colorectal Cancer Model Protocol

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Azoxymethane (AOM, #A5486) was from Millipore Sigma (Temecula, CA, USA) and Dextran sodium sulfate (DSS, mol.wt. 36,000–50,000, #02160110-CF) was from MP Biomedicals (Santa Ana, CA, USA). Antibodies for Ki67 (#ab16667, RRID:AB_302459), CD44 (#ab157107, RRID:AB_2847859), NF-κB (p65) (#ab16502, RRID:AB_443394), ZO-1 (#ab96587, RRID:AB_10680012), Claudin-2 (#ab53032, RRID:AB_869174) and Alcian Blue PAS Stain Kit (#ab245876) were from Abcam (Cambridge, MA). Antibody for β-catenin (#sc-59737, RRID:AB_781850) and Cox-2 (#sc-376861, RRID:AB_2722522) were from Santa Cruz Biotechnology (Dallas, TX, USA). Biotin conjugated secondary anti-rabbit (#E0432, RRID:AB_2313609) and anti-mouse (#E0433, RRID:AB_2687905) antibodies were from Dako/Agilent technologies (West Cedar Creek, TX, USA) and Streptavidin-HRP (#434323, RRID:AB_2619743) and CAS-Block (#008120) were from ThermoFisher Scientific (Waltham, MA, USA). The diaminobenzidine (DAB) kit (#SK4100, RRID:AB_2336382) was from Vector Laboratories (Burlingame, CA, USA). The Proteome Profiler Mouse XL Cytokine Array kit (#ARY028) was purchased from R&D systems (Minneapolis, MN, USA).

Kumar R., Maurya A.K., Parker K.D., Kant R., Ibrahim H., Kabir M.I., Kumar D., Weber A.M., Agarwal R., Kuhn K.A., Ryan E.P, & Raina K. (2022). Gender-based effect of absence of gut microbiota on the protective efficacy of Bifidobacterium longum-fermented rice bran diet against inflammation-associated colon tumorigenesis. Molecular carcinogenesis, 61(10), 941-957.

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Alcian Blue PAS Staining of Colon Tissue

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The paraffin sections of colon tissue undergone gradient dehydration were stained with the Alcian Blue PAS Stain Kit (Abcam, ab245876). According to the manufacturer's instructions, Acetic Acid Solution (3%), Alcian Blue (pH 2.5) solution, Periodic Acid Solution, Schiff's Solution, and Hematoxylin were sequentially applied to tissue sections. Finally, the paraffin sections were dehydrated through graded alcohols and mounted in synthetic resin. The Aperio VERSA 8 (Leica) multifunctional scanner was used to acquire images.

Yu K., Guo Y.Y., Liuyu T., Wang P., Zhang Z.D., Lin D, & Zhong B. (2023). The deubiquitinase OTUD4 inhibits the expression of antimicrobial peptides in Paneth cells to support intestinal inflammation and bacterial infection. Cell Insight, 2(3), 100100.

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Synthesis and Characterization of HA-DBCO Hydrogels

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Laboratory-grade hyaluronic acid (HA) sodium salt (1500–1750 kDa) was purchased from Contipro a.s. (Dolní Dobrouč, Czech Republic). Sulfo-Dibenzocyclooctyne-PEG₄-amine (Sulfo DBCO-PEG4-NH2) was bought from Click Chemistry Tools (Scottsdale, AZ). N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide (EDC), N-hydroxysuccinimide (NHS), azide-terminated poly(N-isopropylacrylamide) (pNIPAM-N₃; 15 kDa), hyaluronidase from bovine testes (Type VI-S), hydrogen peroxide (30% w/w), toluidine blue, Dulbecco’s Modified Eagle’s Medium—high glucose (DMEM), embryoMax L-glutamine solution (100X), foetal bovine serum (FBS), and the antibiotic–antimycotic solution (100X) were purchased from Sigma-Aldrich (St Louis, MO, USA). Dialysis membranes (Biotech CE Dialysis Tubing 300 kDa) were acquired from Repligen (Waltham, MA, USA). The Alcian blue PAS stain kit was purchased from Abcam (Cambridge, UK). Belotero Balance^® and Belotero Volume^® were purchased from Merz Pharma (Geneva, Switzerland) and used as reference products.

Gou S., Porcello A., Allémann E., Salomon D., Micheels P., Jordan O, & Kalia Y.N. (2023). Injectable Hyaluronan-Based Thermoresponsive Hydrogels for Dermatological Applications. Pharmaceutics, 15(6), 1708.

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