The hUC-MSCs were prepared as single-cell suspensions and stained for 30 min at room temperature. PD-L1-FITC antibody (BD Pharmingen, 558065), IFNGR1-PE antibody (BD Pharmingen, 558937), FITC isotype control antibody (BD Pharmingen, 555742), or PE isotype control antibody (BD Pharmingen, 555749) were used to quantify the expression of PD-L1 or IFNGR1 in hUC-MSCs. CD29-PE antibody (BD Pharmingen, 555443), CD34-PE antibody (BD Pharmingen, 555822), CD44-PE antibody (BD Pharmingen, 555479), CD45-PE antibody (BD Pharmingen, 555483), CD73-PE antibody (BD Pharmingen, 550257), CD90-PE antibody (BD Pharmingen, 555596), and CD105-PE antibody (BD Pharmingen, 560839) were used to identify hUC-MSCs. Our gating strategy was to select active hUC-MSCs groups. Flow cytometry was performed using a NovoCyte flow cytometer (ACEA Biosciences).
Cd29 pe antibody
Manufactured by BD
Sourced in United States
The CD29-PE antibody is a fluorescently-labeled monoclonal antibody that specifically binds to the CD29 (Integrin beta 1) surface antigen. CD29 is a cell surface molecule involved in cell-cell and cell-matrix interactions. The PE (Phycoerythrin) fluorescent dye allows for the detection and analysis of CD29-positive cells using flow cytometry or other fluorescence-based techniques.
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2 protocols using cd29 pe antibody
1
Characterization of hUC-MSCs Surface Markers
2
Characterizing Third-Generation BMSCs
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The third generation BMSCs were cultured, and when they filled 90% of the bottom of the culture bottle, they were fully digested with 0.25% trypsin (Gibco, United States). Divide the cell fluid into four tubes, ensuring that each tube has at least 2 × 105 cells were washed twice with PBS, then CD29-PE antibody (BD Biosciences, United States), CD34-PE antibody (Abcam, Cambridge, United Kingdom) and CD45-PE-Cy antibody (BD Biosciences, United States) were added into the test tube, and blank control was set in the fourth tube. Incubate at room temperature and away from light for 30 min, discard the upper liquid after centrifugation, then add 1.0 mL PBS into the test tube, and use flow cytometry (Beckman coulter, Germany) for analysis.
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