Spike rbd fc protein

Manufactured by Sino Biological

Sourced in Germany

The Spike RBD-Fc protein is a recombinant protein that contains the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein fused to the Fc region of a human immunoglobulin. The core function of this protein is to serve as a research tool for studying the interactions between the SARS-CoV-2 spike protein and its receptor, the human angiotensin-converting enzyme 2 (ACE2).

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Lab products found in correlation

Anti human igg fab 2 goat monoclonal antibody, by Abcam (2 mentions) Hrp conjugated anti human fc antibody, by Merck Group (2 mentions) Recombinant human coronavirus sars cov 2 spike glycoprotein s1, by Abcam (1 mentions) Ace2 his protein, by Sino Biological (1 mentions) Recombinant igg1 fc protein, by R&D Systems (1 mentions)

Spelling variants of this product

RBD protein (22 citations) Spike protein (11 citations) RBD-Fc (5 citations) Spike-Fc (1 citations)

2 protocols using spike rbd fc protein

SARS-CoV-2 Spike RBD-Fc Protein Assay

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The following human recombinant proteins and antibodies were used: human SARS-CoV-2 (2019-nCoV) chimeric Spike RBD-Fc protein (Sino Biological, 10108-H08H, Eschborn, Germany); human recombinant IgG1 Fc protein (R & D Systems, 110-HG, Minneapolis, MN, USA); human Platelet Factor 4 (Creative Biomart, PF4-52H, Shirley, NY, USA).
HRP conjugated anti-human Fc antibody (Sigma, AP113P, St. Louis, MO, USA); anti-human IgG (Fab’)2 goat monoclonal antibody (Abcam, ab98535, Cambridge, UK); rabbit polyclonal anti-PF4 antibody (Prodotti Gianni, ab9561, Milano, Italy).

Passariello M., Vetrei C., Amato F, & De Lorenzo C. (2021). Interactions of Spike-RBD of SARS-CoV-2 and Platelet Factor 4: New Insights in the Etiopathogenesis of Thrombosis. International Journal of Molecular Sciences, 22(16), 8562.

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SARS-CoV-2 Spike Protein Binding Assay

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The following human recombinant proteins were used: SARS-CoV-2 (2019-nCoV) chimeric Spike RBD-Fc protein, SARS-CoV-2 (2019-nCoV) Spike RBD-His protein and ACE2 His Protein (all from Sino Biological, 10108-H08H Düsseldorfer Eschborn, Germany); Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (from Abcam, ab273068, Cambridge UK); Recombinant ACE-2 Fc chimeric protein (Z03484 GenScript, Piscataway NJ); recombinant IgG1 Fc protein (R & D Systems, 110-HG Minneapolis MN).
The following antibodies were used: HRP conjugated anti-His antibody (Qiagen, 1014992 Hilden, Germany); HRP conjugated anti-cmyc antibody (Milteny Biotec, 130-092-113 GmbH Friedrich-Ebert-Strabe Germany); HRP conjugated anti-human Fc antibody (Sigma, AP113P USA); anti-human IgG (Fab’)2 goat monoclonal antibody (Abcam, Cambridge UK ab98535).

Passariello M., Gentile C., Ferrucci V., Sasso E., Vetrei C., Fusco G., Viscardi M., Brandi S., Cerino P., Zambrano N., Zollo M, & De Lorenzo C. (2021). Novel human neutralizing mAbs specific for Spike-RBD of SARS-CoV-2. Scientific Reports, 11, 11046.

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