Plan neofluar 40 1.3 na oil dic objective

HeLa cells were seeded into 35-mm Petri dishes and incubated for 24 h. The cell culture medium was removed and the DMEM containing the functional SWCNTs was added into each well, followed by incubation at 37 °C for 2 h. After incubation, HeLa cells were washed by changing the fresh DMEM and then characterized by confocal microscopy with a commercial laser scanning microscope (LSM 510/ConfoCor 2) combination system (Zeiss, Jena, Germany) equipped with a Plan-Neofluar 40×/1.3 NA Oil DIC objective. The excitation wavelength and detection filter settings for each of the fluorescence indicators were as follows. FITC was excited at 488 nm with an Ar-ion laser, and the fluorescence emission was recorded through a 500–530 nm band-pass filter. PPa was excited at 633 nm with a He-Ne laser, and the fluorescence emission was recorded through a 650 nm long-pass filter.

H1299 lung adenocarcinoma cells transfected with or without Flag-hnRNPK were treated with TRAIL (20 ng/ml). Immunofluorescence was performed as described previously34 (link)60 (link). The primary antibodies (1:100 dilution) of hnRNPK, Flag and GSK3β, and the Alexa Fluor 488 and Alexa Fluor 594 conjugated secondary antibodies (ZSGB-BIO, China) were used for observation. DAPI staining was used to determine the morphology of cell nuclei.
The imaging experiments were carried out on laser scanning confocal microscopes (LSM700, Zeiss, Jena, Germany) equipped with a Zeiss Plan-Neofluar 40×/1.3 NA Oil Dic objective as described previously34 (link)60 (link).

To study the integrin targeting property of DT-PNs, 4T1 cells and MCF-7 cells were incubated in a serum-free medium containing DT-PNs for 2 h and then rinsed with PBS and replaced with fresh cell medium, respectively. To further validate that the cancer targeting property of DT-PNs based on the decoration of cRGD moieties, preblocking experiments were designed with cells’ incubation with 2 μM free cRGD for 30 min before their incubation with DT-PNs. The cells were imaged by a commercial laser scanning microscope (LSM 510/ConfoCor 2) combination system (Zeiss, Jena, Germany), equipped with a Plan-Neofluar 40 × /1.3 NA Oil DIC objective. The channel of RhB-labelled DT-PNs was recorded at the excitation wavelength of 543 nm, and the fluorescence emission was recorded by a LP590 nm filter.