Neuroimaging procedures were previously described (17 (link)) and are repeated in detail below to maintain continuity of methods descriptions within a study. At 30 d of age, pigs were subjected to MRI scanning procedures. Before scanning, pigs were anesthetized via an intramuscular injection of Telazol (0.07 mg/kg body weight; Zoetis). Pigs were immediately transferred to the MRI machine in which they were maintained on an average of 2% isoflurane:98% oxygen for the duration of the 60-min scan. Pig heart rate and peripheral capillary oxygen saturation were monitored throughout the duration of the scanning session with the use of an MRI-compatible pulse oximeter. MRI procedures, along with postimaging analyses of brain region volume and diffusion tensor imaging, were previously described (21 (link)). Pigs were scanned with the use of a Siemens MAGNETOM Trio 3T Imager with a Siemens 12-channel head coil. Upon completion of MRI scans, pigs were allowed to recover from anesthesia, vital signs were monitored every 15 min until complete recovery, and pigs were subsequently transported back to the rearing facilities.
Magnetom trio 3t imager
Manufactured by Siemens
The MAGNETOM Trio 3T Imager is a magnetic resonance imaging (MRI) system developed by Siemens. It operates at a magnetic field strength of 3 tesla, providing high-quality imaging capabilities for clinical and research applications.
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4 protocols using magnetom trio 3t imager
1
Pig Brain Imaging at 30 Days
2
Magnetic Resonance Imaging of Developing Piglet Brains
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At 29 (replicate 1) and 31 (replicates 2 and 3) days of age, piglets were subjected to magnetic resonance imaging (MRI) procedures. Scanning procedures were implemented on a Siemens MAGNETOM Trio 3 T Imager using a 32-channel head coil. Upon arrival to the Biomedical Imaging Center located in the Beckman Institute for Advanced Science and Technology, piglets were anesthetized via intramuscular injection of Telazol (0.07 mg/kg body weight; Zoetis, Florham Park, NJ, USA). Once sedated, piglets were transferred to the MRI scanner and maintained on 2% isoflurane/98% oxygen (Isoflurane, Piramal Healthcare, Bethlehem, PA, USA) for the entirety of the 60-min scan. Piglet vital signs were monitored throughout the scan using an MRI-compatible pulse oximeter, and upon completion of the scan, piglets were maintained under anesthesia and immediately euthanized for tissue collection. Detailed methods for manual brain segmentation, volumetric assessment, voxel-based morphometry, and diffusion tensor imaging were previously described (15 (link), 16 (link)).
3
Magnetic Resonance Imaging in Piglet Neurodevelopment
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At 30 days of age, piglets underwent magnetic resonance imaging (MRI) procedures. Piglets were scanned on a Siemens MAGNETOM Trio 3T Imager using a Siemens 32-channel head coil. Upon arrival to the Beckman Institute Biomedical Imaging Center, piglets were anesthetized via intramuscular injection of Telazol (0.07 mg/kg body weight; Zoetis, Florham Park, NJ, USA). Once sedated, piglets were transferred to the MRI scanner and maintained on 2% isoflurane/98% oxygen for the entirety of the 60 min scan. An MRI-compatible pulse oximeter was used to monitor piglet vital signs throughout the scan. Upon completion of the scan, vital signs were monitored and recorded until complete recovery from anesthesia. Specific details regarding piglet imaging sequences and post-imaging analysis of diffusion tensor imaging (DTI) and voxel-based morphometry were previously described (18 (link), 20 (link)).
4
Piglet Brain Metabolites via MRI
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Piglets were subjected to MRI procedures at 30 d-of-age, as described previously.11 (link) All scans were acquired using a Siemens MAGNETOM Trio 3T Imager using a Siemens 32-channel head coil. Magnetic resonance spectroscopy (MRS) was used to non-invasively quantify metabolites both in the hippocampi and intervening tissue, as described previously.13,14 (link) In short, an MRS spin-echo chemical shift sequence was used to assess a voxel which centered over the left and right dorsal hippocampi with a size of 12 mm × 25 mm × 12 mm. Sequence parameters used for MRS data acquisition are as follows: TR = 3000 ms; TE = 30 ms; signal averages = 128 (water-suppressed scan) and 8 (non-water supressed scan); vector size = 1024 point. Water-suppressed and non-water-suppressed data were acquired in institutional units, and all MRS data were analyzed using LC Model (version 6.3), using methods described previously.13,14 (link) Only neurometabolites for which a single data point was obtained for all piglets on the study were analyzed. Thus, neurometabolites in this analysis include: creatine + phosphocreatine (Cr+Pcr), glycerophosphocholine + phosphocholine (GPC+PCh), myo-inositol (mI), n-acetylaspartate (NAA), n-acetylaspartate + n-acetylaspartylglutamate (NAA+NAAG).
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