Infinitylab poroshell 120 ec c18 2.7 μm

The analysis of AMAC-labeled disaccharides was performed on a Vanquish Flex UHPLC System (Thermo Fisher Scientific) coupled with TSQ Fortis triple-quadrupole mass spectrometry as the detector. The C18 column (Agilent InfinityLab Poroshell 120 EC-C18 2.7  μm, 4.6 × 50  mm) was used to separate the AMAC-labeled disaccharides. Buffer A was 50  mM ammonium acetate in water and buffer B is methanol. The elution gradient was from 5–45% buffer B in 10  min, followed by 100% buffer B in 4  min, at a flow rate of 0.3  ml/min. Online triple-quadrupole mass spectrometry operating in the multiple-reaction-monitoring (MRM) mode was used as the detector. The ESI-MS analysis was operated in the negative-ion mode using the following parameters: Neg ion spray voltage at 4.0 kV, sheath gas at 45 Arb, aux gas 15 arb, ion transfer tube temp at 320  °C, and vaporizer temp at 350  °C. TraceFinder software was applied for data processing.

The analysis of AMAC-labeled disaccharides was performed on a Vanquish Flex UHPLC System (Thermo Fisher Scientific) coupled with TSQ Fortis triple-quadrupole mass spectrometry as the detector. The C18 column (Agilent InfinityLab Poroshell 120 EC-C18 2.7 μm, 4.6 × 50 mm) was used to separate the AMAC-labeled disaccharides. Mobile phase A was 50 mM ammonium acetate in water. Mobile phase B is methanol. The elution gradient from 5 to 45% mobile phase B in 10 min, followed by isocratic 100% mobile phase B in 4 min and then isocratic 5% mobile phase B in 6 min, was performed at a flow rate of 0.3 ml/min. Online triple–quadrupole mass spectrometry operating in the multiple-reaction-monitoring (MRM) mode was used as the detector. The ESI–MS analysis was operated in the negative-ion mode using the following parameters: Neg ion spray voltage at 4.0 kV, sheath gas at 45 Arb, aux gas 15 arb, ion transfer tube temp at 320 °C, and vaporizer temp at 350 °C. TraceFinder software was applied for data processing.