Hgcl2

Manufactured by Thermo Fisher Scientific

Sourced in Belgium, United States

HgCl2 is a chemical compound that is commonly used in various laboratory applications. It is a white, crystalline solid with a distinct metallic taste. HgCl2 is soluble in water and has a high thermal stability.

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Lab products found in correlation

5 protocols using hgcl2

Assessing Pseudomonas Heavy Metal Resistance

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To test the resistance of Pseudomonas strains to mercury, the methods of Sone et al. (2013) (link) were employed. Briefly, Pseudomonas strains were grown overnight in 30 mL LB, shaking, at 37°C. 200 μL of the overnight culture was suspended in 3 mL LB medium containing HgCl₂ (Acros Organics; Geel, Belgium) at varied concentrations. Each concentration/strain was cultured in triplicate. Following incubation for 16 h at 37°C, each culture’s absorbance at A₆₀₀ was measured. The same approach was taken for testing the resistance of Pseudomonas strains to copper: 200 μL of the overnight culture was suspended in 3 mL LB medium containing CuSO₄ 5H₂O (Acros Organics; Geel, Belgium) at varied concentrations. Each concentration/strain was cultured in triplicate for 16 h at 37°C and each culture’s absorbance at A₆₀₀ was measured.

Batrich M., Maskeri L., Schubert R., Ho B., Kohout M., Abdeljaber M., Abuhasna A., Kholoki M., Psihogios P., Razzaq T., Sawhney S., Siddiqui S., Xoubi E., Cooper A., Hatzopoulos T, & Putonti C. (2019). Pseudomonas Diversity Within Urban Freshwaters. Frontiers in Microbiology, 10, 195.

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Trace Metal Isotope Analysis Protocol

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Anhydrous CuCl₂ (Acros Organics, Geel, Belgium), HAuCl₄ (Acros Organics, Geel, Belgium), HgCl₂ (Acros Organics, Geel, Belgium), AgF (Acros Organics, Geel, Belgium), FeCl₃ (Acros Organics, Geel, Belgium), NiCl₂ (Acros Organics, Geel, Belgium), CoCl₂ (Acros Organics, Geel, Belgium), NaMoO₄ (Sigma-Aldrich), and KCl (Sigma-Aldrich) were stored in a desiccator under Ar₂. H₂¹⁸O was obtained from Cambridge Isotope Laboratories, Inc. (Andover, MA, USA), and ^18,18O₂ was obtained from Sigma-Aldrich. Ar₂, ^16,16O₂, and chemically pure (CP)-grade CH₄ were obtained from Airgas USA LLC. High-performance liquid chromatography (HPLC)-grade acetonitrile, methanol, and other reagents/chemicals were purchased from Fisher Scientific and used without additional purification. Dianion HP-20 was purchased from Sigma-Aldrich LLC.

Dershwitz P., Bandow N.L., Yang J., Semrau J.D., McEllistrem M.T., Heinze R.A., Fonseca M., Ledesma J.C., Jennett J.R., DiSpirito A.M., Athwal N.S., Hargrove M.S., Bobik T.A., Zischka H, & DiSpirito A.A. (2021). Oxygen Generation via Water Splitting by a Novel Biogenic Metal Ion-Binding Compound. Applied and Environmental Microbiology, 87(14), e00286-21.

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Synthesis of Mercury(II) Complexes with N-Donor Ligands

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The reagents HgCl₂ and HgBr₂ were purchased from Acros Organics (Themo Fisher Scientific, NJ, USA) and HgI₂ from Aldrich Chemistry Co. (Milwaukee, WI, USA). The solvents CH₃OH (99.5%) and CH₃CH₂OH (99.5%) were purchased from Echo Chemical Co., Ltd. (Toufen, Miaoli, Taiwan). The L¹, L², and L³ ligands were prepared according to published procedures with slight modification [27 (link),28 (link),29 (link)].

Govindaraj M., Huang W.C., Lee C.Y., Lakshmanan V., Liu Y.H., So P.B., Lin C.H, & Chen J.D. (2022). Structural Diversity of Mercury(II) Halide Complexes Containing Bis-pyridyl-bis-amide with Bulky and Angular Backbones: Ligand Effect and Metal Sensing. International Journal of Molecular Sciences, 23(14), 7861.

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Bacterial Strains and Growth Media

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Stenotrophomonas maltophilia Oak Ridge strain 02 (ATCC #53510) was purchased from the American Type Culture Collection (Manassas, VA, USA), Enterobacter sp. YSU was described previously [29 (link)] and StrataClone SoloPack Competent Cells and the plasmid, pSC-A-amp/kan, were purchased from Agilent (Santa Clara, CA, USA) as components of the StrataClone PCR (Polymerase Chain Reaction) Cloning Kit. TransforMax™ EC100D™ pir-116 Escherichia coli (E. coli) was purchased from Lucigen (Middleton, WI, USA) [30 (link)].
Lennox LB medium was purchased from Amresco (Solon, OH, USA), and R3A-tris medium was described previously [29 (link)]. When required, LB or R3A-tris medium was supplemented with 100 µg/mL ampicillin (Amresco), 50 µg/mL kanamycin (Amresco) and varying concentrations of HgCl₂, ZnCl₂, CdCl₂, CuSO₄ (Fisher Scientific, Fair Lawn, NJ, USA) and HAuCl₄·3H₂O (Amresco).

Baya G., Muhindi S., Ngendahimana V, & Caguiat J. (2021). Potential Whole-Cell Biosensors for Detection of Metal Using MerR Family Proteins from Enterobacter sp. YSU and Stenotrophomonas maltophilia OR02. Micromachines, 12(2), 142.

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Synthesis of Metal-Organic Compounds

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Methyl acrylate (SD Fine Chemicals, >99%),
potassium bromate (Merck, >99.0%), sodium hydrogen sulfite (Merck,
58.5–67.4%), acrylic acid (AA, Merck, >99.0%), sodium chloride
(NaCl, Qualigens, >99.9%), hydrazine hydrate (SD Fine Chemicals,
99%),
tetra-n-butylammonium bromide (TBAB, Merck, ≥98.0),
silver nitrate (AgNO₃, Qualigens, 99%), dansyl chloride
(DC, Alfa Aesar, 97%), dimethyl sulfoxide (DMSO, SRL-chemicals), HAuCl₄ (Sigma-Aldrich, 99.99%), CuSO₄·5H₂O (Fisher Scientific, 98.5%), Co(NO₃)₂ (Qualigens,
>99.9%), CdCl₂ (Qualigens, >95%), HgCl₂ (Fisher
Scientific, 99.9%), SnCl₄ (Fisher Scientific, 99.9%), FeSO₄·7H₂O (Fisher Scientific, 99.9%), TiO₂ (Qualigens, >99.9%), NiCl₂ (Qualigens, >99.9%),
Cr₂(SO₄)₃ (Qualigens, >99.9%),
MgSO₄ (Merck, 99%), PbCl₂ (Qualigens, >99.9%),
ZnSO₄·7H₂O (Qualigens, >99.0%), platinum
bromide
(PtBr₂, Sigma-Aldrich, 98%), and cesium chloride (CsCl₃·7H₂O, Qualigens, >99.9%) were used without
further purification. Tetrahydrofuran (THF, Qualigens, 99.0%) was
refluxed over sodium metal and benzophenone overnight and distilled
under a nitrogen atmosphere prior to use. Water used for the preparation
of aqueous solution was obtained by deionization and filtration with
a Millipore Elix-10 purification apparatus. The electrical conductivity
of deionized water is 0.0054 mS/cm.

Ujjwal R.R., Sona C., Debnath S., Yadav P.N, & Ojha U. (2017). Dye-Labeled Polyacryloyl Hydrazide–Ag Nanoparticle Fluorescent Probe for Ultrasensitive and Selective Detection of Au Ion. ACS Omega, 2(8), 4278-4286.

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