Ots514 s7652

Antibodies for PBK (ab75987), ATG7 (ab52472), LC3B (ab51520), p-ERK1/2 (ab72699), ERK1/2 (ab17942), and EVI1 (ab124934) were from Abcam (Cambridge, UK); β-actin (A5441), CDDP (PHR1624), Mitomycin C (M4287), Baf A1 (196000), and CQ (C6628) were all purchased from Sigma-Aldrich (St. Louis, MO, USA); antibodies for poly (ADP-ribose) polymerase (PARP, 9542S), Caspase-3 (29629S), Cleaved-caspase-3 (9664S), mTOR (2972S), p-mTOR (5536S), p-p70S6K (9206S), p70S6K (9202S), p-4EBP1 (9459S), 4EBP1 (9452S), DYKDDDDK Tag (Flag, 14793S) and Epithelial-Mesenchymal Transition (EMT) Antibody Sampler Kit (9782) were obtained from Cell Signaling Technology (Danvers, MA, USA). U0126 (S1102) and OTS514 (S7652) were purchased from Selleck Chemicals (Houston, TX, USA).

Antibodies against GST (10000-0-AP), poly (ADP-ribose) polymerase 1 (PARP1, 13371-1-AP), Flag (20543-1-AP), MYC-tag (16286-1-AP), GFP (66002-1-Ig), Ki-67 (27309-1-AP), PBK (16110-1-AP), XIAP (10037-1-Ig), Bcl-XL (26967-1-AP), Lamin B1 (12987-1-AP), p65 (66535-1-Ig), and caspase 3 (19677-1-AP) were purchased from Proteintech (Wuhan, China); β-actin (A5441) was obtained from Sigma-Aldrich (St. Louis, MO, USA); The mouse IgG (A7028) and rabbit IgG (A7016) were purchased from Beyotime (Shanghai, China). Antibodies for IκBα (ab32518), TRIM37 (ab264190), and GAPDH (ab8245) were obtained from Abcam (Cambridge, UK). Cleaved caspase 3 (9661) and p-IκBα (2859) were obtained from Cell Signaling Technology (Danvers, MA, USA). p-Thr (sc-5267), p-Ser (sc-81514), and p-Tyr (sc-7020) were purchased from Santa Cruz (Dallas, TX, USA). Goat anti-mouse IgG Alexa Fluor-594 (A-11030) for immunofluorescence staining was obtained from Invitrogen (Waltham, MA, USA). Olaparib (AZD2281), JSH-23 (S7652), and OTS514 (S7652) were purchased from Selleck Chemicals (Houston, TX, USA).