Laboratory Chinchilla rabbits with an age of 6.5 ± 0.2 months and bodyweight of 4.5 ± 0.6 kg were used in this work. All manipulations of the rabbits complied with the European Convention of laboratory animal protection [26 (link)] and were approved by the Local Ethical Committee of NMRC MH RF (NCT02255188 from 16 January 2014). After the autopsy, the iliac artery was excised and washed with PBS. The stent mounted on balloons (SINUS coronary stents 3.5 × 18 mm, Angioline, Novosibirsk, Russia) was introduced in the artery and was positioned so that the artery completely covered the stent. The stent, covered with the artery, was placed in a through-hole 2.5 mm in diameter drilled into a 5 mL tube (Corning, NY, USA) close to the bottom. The balloon was dilated with a basic COMPAC inflation device. Then, 1 mL of PBS or BP was added in a tube at time-point zero. The samples were incubated at 25 °C and aliquots of 200 µL were taken from different time intervals (20 min, 60 min, 3 h, 9 h, and 27 h) to calculate the radioactivity at each time point, and an additional 200 µL of fresh medium was introduced in a tube with subsequent incubation until the next time point.
5 ml tube
Manufactured by Corning
Sourced in United States
The 5 mL tube is a laboratory equipment item designed to hold and contain liquid samples up to 5 milliliters in volume. It is a standardized container made of a durable material, typically plastic or glass, to provide a secure and reliable storage solution for various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 5 ml tube
1
In Vitro Stent Release Kinetics
2
Flow Cytometry Immunophenotyping of Murine Immune Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were adjusted to 1 Â 10 7 cells/mL and 100 mL was transferred to a 5-mL tube (Corning Life Sciences). Cells were then preincubated with 2-mL mouse Fc block anti-mouse CD16/CD32 mAb (No. 553142, BD Biosciences) for 5 minutes at 4 C. Antibodies of interest were then directly added to cells at a final concentration of 10 mg/mL in the presence of mouse Fc block solution and incubated at 4 C in the dark for 30 minutes. The antibodies used were listed in Supplementary Table S1 and were obtained from BD Biosciences (CD4, CD8, CD25) or BioLegend (CD45, CD335, CD11b, Ly6C, Ly6G, Gr-1, F4/80). After incubation, cells were washed three times in 2-mL flow staining buffer at 400 Â g at 4 C. After the final wash, cells were resuspended in 400-mL FluoroFix buffer (BioLegend) and incubated in dark for 30 minutes at room temperature. Fixed cells were then washed in flowstaining buffer and stored in the dark at 4 C until analysis on BD LSRFortessa X-20 Cell Analyzer.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!