The mouse melanoma cell line B16F10 was purchased from RIKEN Cell Bank (Tsukuba, Japan), and NHEMs were purchased from KURABO (Osaka, Japan). RPMI-1640, l -DOPA, α-MSH, U 0126, and BIO were from Sigma-Aldrich (St. Louis, MO, USA). The DermaLife® Basal Medium of Lifeline (MD, USA) and the DermaLife® M LifeFactors kit were used together as the medium for the NHEMs. α-mangostin was purchased from Wako (Osaka, Japan). Trypan blue (0.4%) was from Logos Biosystem (Gyeonggi-do, South Korea). Antibodies against tyrosinase (Santa Cruz Biotechnology, Dallas, TX, USA), TRP-2 (Santa Cruz Biotechnology), MITF, ERK, p-ERK, GSK3β, p-GSK3β, β-actin (Cell Signaling Technology, Danvers, MA, USA) were also obtained.
α-mangostin was dissolved in dimethyl sulfoxide (DMSO) for B16F10 cells and 70% EtOH for NHEMs. The adjusted α-mg sample was sterilized using a 0.20 μm filter and stored at −80 °C.
α-mangostin was dissolved in dimethyl sulfoxide (DMSO) for B16F10 cells and 70% EtOH for NHEMs. The adjusted α-mg sample was sterilized using a 0.20 μm filter and stored at −80 °C.