HUVEC were purchased from (Lonza) and were maintained using EGM2 medium (Lonza). HUVEC were used between passages 4 and 5. Jurkat T cells (E6.1, ATCC) and the corresponding CD47-deficient Jurkat mutant JinB8 were provided by Dr. Eric Brown, Genentech (Reinhold et al., 1999 (link)) and were maintained at 2.0 × 105 cells per ml in RPMI 1640 medium (Life Technologies) supplemented with 10% FBS, glutamine, penicillin, and streptomycin. Jurkat cells were maintained for a maximum of 4 weeks for experiments. Human anti-CD3 and anti-CD47-FITC (BD Biosciences), anti-CD69 (R&D systems), anti-actin, anti-tubulin, anti-VEGFR2, anti-VEGFR2 Y1175 (Cell Signaling), anti-CD47 (B6H12, Abcam) and azide-free functional grade anti-CD47 (e-Biosciences) were purchased from the indicated vendors.
Human anti cd3 and anti cd47 fitc
Manufactured by BD
Human anti-CD3 and anti-CD47-FITC is a laboratory reagent used for the detection and analysis of CD3 and CD47 cell surface markers. It is a fluorescently labeled antibody that binds specifically to these targets, allowing their identification and quantification using flow cytometry or other immunoassay techniques.
Automatically generated - may contain errors
Lab products found in correlation
Anti tubulin, by Cell Signaling Technology (2 mentions)
Human umbilical vein endothelial cells (huvec), by Lonza (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Anti actin, by Cell Signaling Technology (2 mentions)
Anti vegfr2, by Cell Signaling Technology (2 mentions)
Anti cd69, by R&D Systems (2 mentions)
Anti vegfr2 y1175, by Cell Signaling Technology (2 mentions)
Anti cd47 b6h12, by Abcam (2 mentions)
Anti cd47, by Thermo Fisher Scientific (2 mentions)
Egm 2 medium, by Lonza (2 mentions)
2 protocols using human anti cd3 and anti cd47 fitc
1
HUVEC and Jurkat Cell Maintenance
2
HUVEC and Jurkat Cell Maintenance
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