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Sucrose octasulfate sos sodium salt

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Sucrose octasulfate (SOS) sodium salt is a chemical compound that functions as an organic sulfate. It is commonly used as a laboratory reagent for various applications in the scientific research and analysis field.

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2 protocols using sucrose octasulfate sos sodium salt

1

Irinotecan-loaded Liposomal Delivery System

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Tetraethylorthosicate (TEOS), triethanolamine, cetyltrimethylammonium chloride solution (CTAC, 25 wt% in water), triethylamine (TEA), Dowex 50WX8 resin, Sepharose CL-4B, and Sephadex G-25 were purchased from Sigma-Aldrich, USA. 1,2-Distearoyl-sn-glycero-3-phosphocholine (DSPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-PEG2000), and cholesterol (Chol) were purchased from Avanti Polar Lipids, USA. Sucrose octasulfate (SOS) sodium salt was purchased from Toronto Research Chemicals, Inc, Canada. Irinotecan hydrochloride trihydrate was purchased from LC Laboratories, USA. Penicillin, streptomycin, and Dulbecco’s modified Eagle medium (DMEM) were obtained from Invitrogen. Fetal bovine serum (FBS) was purchased from Gemini Bio Products. A rabbit mAb antibody (catlog. #9664) that detects activated (cleaved) caspase-3 was purchased from Cell Signaling. Matrigel Matrix Basement Membrane was purchased from BD Bioscience. Centrifugal filter units (cutoff size: 10K & 30K) were purchased from EMD Millipore. All chemicals were directly used without further purification.
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2

Nanoparticle-based drug delivery system

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Tetraethylorthosilicate, triethanolamine, cetyltrimethylammonium chloride solution (25 wt% in water), triethylamine (TEA), HCQ, Sepharose CL-4B matrix, and crystal violet reagent were purchased from Sigma-Aldrich. DSPC, DSPE-PEG2000, and Chol were purchased from Avanti Polar Lipids. Sucrose octasulfate (SOS) sodium salt was obtained from Toronto Research Chemicals, Inc., and converted to sucrose octasulfate triethylamine salt (TEA8SOS) through an ion exchange chromatography process24 (link),26 (link). PAL was purchased from LC Laboratories. ABT-737 was purchased from AdooQ Bioscience. Matrigel matrix basement membrane was purchased from BD Bioscience. Premo™ autophagy tandem sensor RFP-GFP-LC3B Kit, Tali cell cycle kit, and CellROX™ Green flow cytometry reagents were purchased from Thermo Fisher Scientific. BrdU cell proliferation assay kit and senescence β-galactosidase staining kit were purchased from Cell Signaling Technology.
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