The Pro-Q Diamond Phosphoprotein Enrichment kit/reagents and NuPAGE precast 4%–12% Bis–Tris polyacrylamide gels were purchased from Life Technologies. Bradford Protein concentration assay kit and TPCK Trypsin 20233 for protein digestion were obtained from Thermo Fisher. Protease and phosphatase (for serine, threonine, and tyrosine) inhibitors and all other reagents were of analytical grade and quality and were obtained from Sigma (if not stated otherwise).
Bradford protein concentration assay kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Bradford Protein concentration assay kit is a colorimetric assay used to determine the concentration of protein in a sample. It is based on the color change of the Coomassie Brilliant Blue G-250 dye in response to different concentrations of protein.
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Lab products found in correlation
2 protocols using bradford protein concentration assay kit
1
Phosphoproteome Enrichment and Analysis
2
Venome Protein Characterization by Bradford Assay and LC-MS/MS
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The protein concentration was determined by the Bradford Protein Concentration Assay Kit (Thermo Fisher Scientific Incorporation, MA, USA), according to the manufacturer’s instructions [46 (link)]. The purified venome protein was separated by SDS-PAGE and identified by LC-MS/MS and a Uniprot database search (https://www.uniprot.org/uniprot/?query=agkistrodon+acutus&sort=score ).
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