Sodium azide

Manufactured by Tianjin Damao

Sourced in China

Sodium azide is a colorless crystalline compound with the chemical formula NaN3. It is commonly used as a laboratory chemical in various applications.

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Lab products found in correlation

Bovine serum albumin (bsa), by Merck Group (1 mentions) Hydrochloric acid (hcl), by Tianjin Damao (1 mentions) Ovalbumin (ova), by Merck Group (1 mentions) Potassium chloride (kcl), by Tianjin Damao (1 mentions) Sodium chloride (nacl), by Tianjin Damao (1 mentions) Goat anti mouse igg, by Merck Group (1 mentions) Acetic acid, by Tianjin Damao (1 mentions) Potassium carbonate, by Tianjin Damao (1 mentions) Potassium dihydrogen phosphate, by Tianjin Damao (1 mentions) Tween 20, by Tianjin Damao (1 mentions) Zearalenone, by Merck Group (1 mentions) Deoxynivalenol, by Merck Group (1 mentions)

2 protocols using sodium azide

Multiplex Mycotoxin Detection Assay

Cited 1 time

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Methanol and acetonitrile (HPLC grade) were purchased from Merck Drugs and Biotechnology (New York, NY, USA). AFB₁, AFB₂, AFG₁, AFG₂, OTA, zearalenone (ZEN), trichothecenes (T₂), deoxynivalenol (DON), fumonisins (FB) standard solutions, goat anti-mouse IgG, bovine serum albumin (BSA), and ovalbumin (OVA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium borate decahydrate, potassium carbonate, sucrose, Tween-20, acetic acid, sodium azide, sodium chloride, sodium hydrogen phosphate, potassium dihydrogen phosphate, potassium chloride, hydrochloric acid, and sodium bicarbonate were purchased from Damao Chemical Reagent Factory (Tianjin, China). Colloidal gold (particle size ~60 mm) and AFB₁/OTA monoclonal antibody were purchased from Clover Technology Group Inc. (Beijing, China).
The following reagents were used: PBS; boric acid buffer solution (0.2 mol/L); resuspension solution, gold label pad treatment solution, sample pad treatment solution, sample buffer [23 (link)].

Zhao X., Jin X., Lin Z., Guo Q., Liu B., Yuan Y., Yue T, & Zhao X. (2021). Simultaneous Rapid Detection of Aflatoxin B1 and Ochratoxin A in Spices Using Lateral Flow Immuno-Chromatographic Assay. Foods, 10(11), 2738.

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Phenotypic Analysis of RAW264.7 Macrophages

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RAW264.7 cells (2x10⁶ cells/ml) were cultured for 24 h in the presence of SHP (100 µg/ml) or LPS (1 µg/ml); cells treated with RPMI were used as the negative control group and LPS as the positive control group. Subsequently, cells were harvested and washed with FACS buffer containing 1% bovine serum albumin and 0.1% sodium azide (Damao Chemical Reagent Factory). Cells were then stained with 10 µg/ml anti-mouse CD40 conjugated with APC (cat. no. ab272271) and CD11b conjugated with FITC (cat. no. ab24874) (both from Abcam) for 30 min at 4˚C Parallel sets of cells were incubated without the antibodies and their autofluorescence intensity served as a non-specific negative control. A total of 100,000 viable cells per treatment (as determined using light scatter profiles) were analyzed using a FACS Symphony A5 ﬂow cytometer (BD Biosciences) and CytExpert cytometry software (version 1.0) (Beckman Coulter, Inc.).

Wang M., Fu C., Zhang M., Zhang Y, & Cao L. (2022). Immunostimulatory activity of soybean hull polysaccharide on macrophages. Experimental and Therapeutic Medicine, 23(6), 389.

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