After single cell preparation from organoids as described above, cells were resuspended in 200 µl of 1× binding buffer and 1 μl of APC Annexin V from apoptosis detection Kit from BD Bioscience following incubation at RT for 15 min. After washing with 1× binding buffer, cells were resuspended in 1× binding buffer (100 µl) and analyzed using FACSAriaII (BD Biosciences) and data were analyzed using FlowJo software.
Apc annexin 5 from apoptosis detection kit
Manufactured by BD
The APC Annexin V from the apoptosis detection Kit is a fluorescent labeling reagent used to detect and quantify cells undergoing apoptosis. It binds to phosphatidylserine, which is exposed on the cell surface during apoptosis. The APC (Allophycocyanin) fluorescent dye allows the detection of apoptotic cells by flow cytometry.
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3 protocols using apc annexin 5 from apoptosis detection kit
1
Annexin V Detection of Apoptosis
2
Annexin V Detection of Apoptosis
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After single cell preparation from organoids as described above, cells were resuspended in 200 µl of 1× binding buffer and 1 μl of APC Annexin V from apoptosis detection Kit from BD Bioscience following incubation at RT for 15 min. After washing with 1× binding buffer, cells were resuspended in 1× binding buffer (100 µl) and analyzed using FACSAriaII (BD Biosciences) and data were analyzed using FlowJo software.
3
Annexin V Detection of Apoptosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After single cell preparation from organoids as described above, cells were resuspended in 200 µl of 1× binding buffer and 1 μl of APC Annexin V from apoptosis detection Kit from BD Bioscience following incubation at RT for 15 min. After washing with 1× binding buffer, cells were resuspended in 1× binding buffer (100 µl) and analyzed using FACSAriaII (BD Biosciences) and data were analyzed using FlowJo software.
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