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2 protocols using ccr1 percp cy5.5 5f10b29

1

Multiparametric Phenotyping of MAIT Cells

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PBMCs were stained with 1:100 MR1 5-OP-RU or 6-FP (as a control) tetramer for 40 min at room temperature. After 40 min, incubation cells were also stained with fixable viability dye eFluor 506 (eBioscience) and with combinations of anti-human CD3-Alexa Fluor 700 (UCHT1; BD Pharmingen), CD4-allophycocyanin-eFluor 780 (RPA-T4; eBioscience), CD8-BV650 (RPA-T8; BioLegend), Vα7.2-PE-Cy7 (3C10; BioLegend), CD161-allophycocyanin (HP-3G10; eBioscience), TIGIT-PerCP-eFluor710 (MBSA43; eBioscience), CXCR6-PE Dazzle594 (K041E5; BioLegend), CCR1-PerCP Cy5.5 (5F10B29; BioLegend), CD243-BV421 (UIC2; BD Biosciences), CXCR4-PE Dazzle 594 (12G5; BioLegend), CD127-FITC (eBioRDR5; eBioscience), CD14-V500 (M5E2; BD Biosciences), CD14-BV421 (HCD14; BioLegend), and CD19-V500 (HIB19; BD Biosciences) for 30 min at room temperature. After two washes in PBS, cells were resuspended in PBS and cells were acquired on a LSRII Cytometer (Becton Dickinson).
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2

Multiparametric Phenotyping of MAIT Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMCs were stained with 1:100 MR1 5-OP-RU or 6-FP (as a control) tetramer for 40 min at room temperature. After 40 min, incubation cells were also stained with fixable viability dye eFluor 506 (eBioscience) and with combinations of anti-human CD3-Alexa Fluor 700 (UCHT1; BD Pharmingen), CD4-allophycocyanin-eFluor 780 (RPA-T4; eBioscience), CD8-BV650 (RPA-T8; BioLegend), Vα7.2-PE-Cy7 (3C10; BioLegend), CD161-allophycocyanin (HP-3G10; eBioscience), TIGIT-PerCP-eFluor710 (MBSA43; eBioscience), CXCR6-PE Dazzle594 (K041E5; BioLegend), CCR1-PerCP Cy5.5 (5F10B29; BioLegend), CD243-BV421 (UIC2; BD Biosciences), CXCR4-PE Dazzle 594 (12G5; BioLegend), CD127-FITC (eBioRDR5; eBioscience), CD14-V500 (M5E2; BD Biosciences), CD14-BV421 (HCD14; BioLegend), and CD19-V500 (HIB19; BD Biosciences) for 30 min at room temperature. After two washes in PBS, cells were resuspended in PBS and cells were acquired on a LSRII Cytometer (Becton Dickinson).
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