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Luperox tbh70x tert butyl hydroperoxide solution tbooh

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Luperox TBH70X is a tert-butyl hydroperoxide solution (tBOOH) produced by Merck Group. It is a clear, colorless liquid that serves as an oxidizing agent and initiator in various chemical reactions and processes.

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Lab products found in correlation

Hydrogen peroxide solution h2o2, by Merck Group (1 mentions) Menadione, by Merck Group (1 mentions) Paraquat dichloride hydrate paraquat, by Merck Group (1 mentions)

Close spelling variants of this product

Tert-butyl hydroperoxide (112 citations) Luperox® TBH70X (19 citations) Tert-Butyl hydroperoxide solution (14 citations) Luperox (12 citations) TBH70X (2 citations)

2 protocols using luperox tbh70x tert butyl hydroperoxide solution tbooh

1

Yeast Stress Response Assay

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Strains were cultured overnight in YPD medium, 10-fold serially diluted, and plated onto YPD medium with or without the following stress agents: 0.04% methyl methanesulfonate (MMS) (Sigma-Aldrich; catalog no. 129925), 0.06% ethidium bromide solution (EtBr) (Sigma-Aldrich; catalog no. E1510), 0.25 mM menadione (Sigma-Aldrich; catalog no. M5625), 0.25 mM paraquat dichloride hydrate (paraquat) (Sigma-Aldrich; catalog no. 36541), 0.4 mM Luperox TBH70X tert-butyl hydroperoxide solution (tBOOH) (Sigma-Aldrich; catalog no. 458139), and 5 mM hydrogen peroxide solution (H2O2) (Sigma-Aldrich; catalog no. 216763). One set of plates was exposed to UV light (120 J m−2). Plates were incubated at 28°C for 2 days.
Boyce K.J., Wang Y., Verma S., Shakya V.P., Xue C, & Idnurm A. (2017). Mismatch Repair of DNA Replication Errors Contributes to Microevolution in the Pathogenic Fungus Cryptococcus neoformans. mBio, 8(3), e00595-17.
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2

Stress Response Profiling of Yeast Strains

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Strains were cultured overnight in YPD medium, ten-fold serially diluted, and plated onto YPD ± stress agents at 28 °C for 2 days: 0.06 % ethidium bromide solution (EtBr) (Sigma Aldrich, E1510), 0.04 % methyl methanesulfonate (MMS) (Sigma Aldrich, 129925), 0.25 mM menadione (Sigma Aldrich, M5625), 5 mM hydrogen peroxide solution (H2O2) (Sigma Aldrich, 216763) and 0.4 mM Luperox TBH70X tert-butyl hydroperoxide solution (tBOOH) (Sigma Aldrich, 458139). One set of plates was exposed to UV light (120 Jm−2) and one set was incubated at 37 °C. Strains were also plated on l-DOPA medium to assess melanization.
Boyce K.J., Cao C., Xue C, & Idnurm A. (2019). A spontaneous mutation in DNA polymerase POL3 during in vitro passaging causes a hypermutator phenotype in Cryptococcus species. DNA repair, 86, 102751.
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