Nano zoomer scan sq1

On the 30th day, 20 mice were anaesthetized as previously described and fixed via cardiac perfusion with 4% paraformaldehyde after flushing out the red blood cells with PBS. The whole brains were isolated and fixed in 4% paraformaldehyde for 1 day. The brains were then dehydrated in ethanol, defatted in xylene, embedded in paraffin, and serially sectioned at 5 μm thicknesses by using a rotary microtome (Leica RM2135, Leica Biosystems, Heidelberg, Germany). Subsequently, the sections were stained with HE according to procedure which has been reported [18 ]. The digital photograph of hippocampal CA1, CA3 and amygdala were observed by Leica DMI4000B (Leica Biosystems, Heidelberg, Germany) and a Hamamatsu Nano Zoomer scan SQ1.0 (Hamamatsu Photonics, Shizuoka, Japan) with NDP, respectively.

The whole brains were isolated and fixed in 4% paraformaldehyde for 24 h and then rinsed in running water for 24 h. Thereafter, each sample was dehydrated using a standard alcohol series, defatted in xylene, and finally embedded in paraffin. Histological sections, 5 μm thick, were dewaxed, hydrated, and stained with HE. After drying, the histological slides were covered with cover slips. Digital photography of the hippocampus and cerebral cortex was performed under a Leica DMI4000B optical microscope (Leica Biosystems, Heidelberg, Germany) and a Hamamatsu NanoZoomer Scan SQ1.0 (Hamamatsu Photonics, Shizuoka, Japan) with NDP.