Cells (2×105/well were plated into 12-well plates until the cells reached 90% confluency. The fused monolayer cells were then scratched with a pipette tip (100 µl), and the exfoliated cells were washed gently with PBS. Subsequently, the cells were cultured in a serum-free medium for 24 h. Using an optic microscope (Leica), the images at 0 and 24 h were captured with ×100 magnification to evaluate cell migration. This experiment was repeated three times.
Optic microscope
Manufactured by Leica
Sourced in Germany
The Optic microscope is a precision instrument designed for magnifying and observing small objects or specimens. It utilizes lenses to produce an enlarged, detailed image of the subject matter. The core function of the Optic microscope is to provide a clear and high-resolution view of the examined sample.
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Lab products found in correlation
3 protocols using optic microscope
1
Wound Healing Assay for Cell Migration
2
Histological Analysis of Tissue Specimens
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For VG, H&E and Masson's trichrome staining, the specimens were fixed in 10% formalin for 2 days, dehydrated with a graded series of ethanol and embedded in paraffin. The samples were sliced into 5 μm thick sections, which were afterwards stained with Van Gieson Staining, Hematoxylin-Eosin and Masson's Trichrome Stain Kit (Solarbio, China), then examined with an optical microscope (Leica). For fluorescence labelling, the specimens were dehydrated in a graded alcohol series for 7 days and then embedded in methyl methacrylate without decalcification. The non-decalcified samples were cut with a diamond saw (SP1600, Leica) and ground to approximately 150 μm in thickness. To observe the fluorescence labelling, the specimens were observed with a confocal laser scanning microscope (Leica). For VG staining, the specimens stained with Van Gieson Staining were observed with an optic microscope (Leica).
3
Isolation and Culturing of Cyanobacteria from Phytoplankton
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Phytoplankton samples, collected in each sampling site, were visualized under an optic microscope (Leica DMLB, Germany) and isolated through the micromanipulation technique using a Pasteur pipette. Both colonial and filamentous cyanobacteria strains belonging to M. aeruginosa, R. raciborskii and P. agardhii were isolated and transferred into culture tubes supplemented of 5 mL of Z8 culture medium to promote cyanobacterial growth [28 ]. Isolated microorganisms were maintained in culture under 25 °C, with a photoperiod of 14 h:10 h light-dark and 25 μEm2s of light intensity without aeration. Cultures were transferred to 50 mL culture flasks with filter caps (Orange Scientific, Braine-l’Alleud, Belgium) containing Z8 culture medium and maintained in the same growth conditions. Cultures were visualized in an Olympus BX41 optic microscope coupled with an Olympus DP72 photograph machine and using Olympus Cell^B software for image acquisition.
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