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Fc blocker cd16 cd32

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The Fc blocker (CD16/CD32) is a laboratory reagent that binds to Fc receptors on cells, blocking Fc-mediated interactions. This product is intended for use in flow cytometry and other cell-based assays to prevent non-specific binding of antibodies to Fc receptors.

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Lab products found in correlation

Facsverse, by BD (1 mentions) Cd19 pe, by BioLegend (1 mentions) Il 10 pe, by BD (1 mentions) B220 apc cy7, by BD (1 mentions) Cd1d bv421, by BD (1 mentions) Cd43 pe cy7, by BD (1 mentions) Cd21 apc cy7, by BioLegend (1 mentions) Cytofix cytoperm kit, by BD (1 mentions) Cd5 pe cy7, by BioLegend (1 mentions) Cd5 percp, by BD (1 mentions) B220 fitc, by BD (1 mentions) Anti mouse igm apc, by BD (1 mentions) Cd93 pe cy7, by BioLegend (1 mentions)

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Fc blocker (46 citations) CD16/CD32 (41 citations)

2 protocols using fc blocker cd16 cd32

1

PBMC Immunophenotyping by Flow Cytometry

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Peripheral blood mononuclear cells (PBMCs) were isolated and incubated with Fc blocker (CD16/CD32; BD Biosciences, San‐Jose, CA) for 15 minutes to avoid nonspecific binding and were stained with a combination of Nile red fluorescein isothiocyanate, phycoerythrin (PE)‐human leukocyte antigen DR isotype (HLA‐DR), PE/cyanine 7/anti‐CD11b, and allophycocanin anti‐CD163 antibodies for 30 minutes at 4°C, washed with phosphate‐buffered saline, and fixed in 0.5% paraformaldehyde. The cells were analyzed on BD FACS VERSE (BD Biosciences).
Maras J.S., Das S., Bhat A., Kumar Vyas A., Yadav G., Chaudhary S., Sukriti S., Gupta A.C., Bihari C., Mahiwall R, & Sarin S.K. (2019). Dysregulated Lipid Transport Proteins Correlate With Pathogenesis and Outcome in Severe Alcoholic Hepatitis. Hepatology Communications, 3(12), 1598-1625.
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2

Multicolor Flow Cytometry of Immune Cells

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The following antibodies were used for staining: anti-mouse IgM-APC, B220-FITC, CD93-PE CF594, CD19-Alexa Fluor 647, IL10-PE, CD23-BB515, CD5-PerCP, CD1d-BV421, CD43-PE Cy7, B220-APC Cy7, Fc Blocker (CD16/CD32), Cytofix/CytoPerm kit (all from BD Pharmingen), CD23-PerCP/Cy5.5, CD93-PE Cy7, CD1d-PB, CD5-PE Cy7, CD21-APC Cy7, CD19-PE, CD43-APC Cy7 (all from Bio Legend), Live/Dead aqua marker (Molecular Probes).
Mondal K., Das S., Naskar K, & Roy S. (2021). Modulation of Splenic B Cell Subsets during Experimental Leishmania donovani Infection in BALB/c Mice. Pathogens, 10(7), 814.
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