Edta k3 coated microvettes

All animal experiments were approved by the committee of animal experimentation of the Veterinary Department of the Canton of Bern, under approval number BE-50/17. C57BL/6J mice were provided by Janvier Labs (France). Sphk2^tm1geno were generated by GenOway S.A. (Lyon, France). C57BL/6J wildtype or Sphk2 knockout mice were injected in the peritoneum with a single dose of 1 mg/kg ST-2191 or DMSO in PBS, and after 24 h, blood was collected from the heart under terminal isoflurane-induced anesthesia in EDTA-K3 coated microvettes (Sarstedt; Sevelen, Switzerland). The number of lymphocytes was measured with a Scil Vet ABC™ Hemocytometer and was expressed per one liter of blood (Scil Animal Care Company GmbH; Viernheim, Germany).

C57BL/6J mice were injected intraperitoneally with 5 mg/kg of ST-1893, ST-1894, or DMSO in PBS, and, after 24 h, blood was collected in EDTA-K3-coated microvettes (Sarstedt; Sevelen, Switzerland) with cardiac puncture under deep terminal isoflurane-induced anesthesia. The number of lymphocytes was assessed with a Scil Vet ABC^TM Hemocytometer (Scil animal care company GmbH; Viernheim, Germany).

All animal experiments were approved by the committee of animal experimentation of the Veterinary Department of the Canton of Bern, under approval number BE-50/17. C57BL/6J mice were provided by Janvier Labs (Le Genest-Saint-Isle, France). Sphk2^tm1geno (Sphk2−/−) were generated by GenOway S.A. (Lyon, France). Blood was collected in EDTA-K3-coated microvettes (Sarstedt, Sevelen, Switzerland) from the tail vein of 8-week old C57BL/6J (Wt) or Sphk2−/− mice and the number of RBC and hemoglobin were assessed with a Scil Vet ABC^TM Hemocytometer (Scil Animal Care Company GmbH; Viernheim, Germany).