Cool2 α pix

Stadie-Riggs tissue slicer was purchased from Thomas Scientific (Swedesboro, NJ); RhoA, Cdc42 and filamentous actin (F-actin) to globular actin (G-actin) (F/G-actin) ratio assay kits were purchased from Cytoskeleton (Denver, CO); the Rho kinase inhibitor Y27632 and the Arp2/3 complex inhibitor CK666 were purchased from EMD Millipore (Billerica, MA); FAK inhibitor FP 573228 and the actin polymerization inhibitors latrunculin A, and cytochalasin D were purchased from Tocris Bioscience (Bristol, UK); All PCR reagents, RNA isolation kit, TURBO DNase, SuperScript II, Lipofectamine 2000 Transfection Reagent as well as phospho-Pyk2(Tyr402) and phospho-paxillin(Tyr118) antibodies were purchased from Thermo Fisher Scientific (Rockford, IL). Anti-Arp2/3, N-WASP antibodies were purchased from Abcam (Cambridge, UK); Cdc42 antibody, phospho-FAK (pY397), and paxillin antibodies were purchased from BD Biosciences; and Cool1/β-Pix, Cool2/α-Pix, DOCK 180, and FAK antibodies were purchased from Cell Signaling Technology (Danvers, MA). Western blot supplies were purchased from Bio-Rad Laboratories (Hercules, CA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO)