Primary antibodies used were: Wdr1 (G-13, Santa Cruz), 1:100; Destrin (ab11072, abcam), 1:100; cofilin (ab42824, abcam), 1:5,000; HPRT (ab10479, abcam), 1:5,000; GAPDH (ab8245, abcam), 1:5,000; GFP (ab13970), 1:2,000; myosin II (PRB-440P, Covance), 1:200; pericentrin (PRB-432C, Covance), 1:1,000; E-cadherin (ECCD-1, M. Takeichi, RIKEN, Japan), 1:500; CD104 (β4 integrin, 553745 BD-Pharmingen), 1:500; P-cadherin (P-cad, Zymed), 1:1,000; Keratin14 (PRB-155P, Covance), 1:1,000; keratin 10 (PRB-159P, Covance), 1:1,000; Celsr1 (Fuchs lab), 1:250; keratin 6 (Rb 415, Millipore), 1:500; PAR3 (07-330, Millipore), 1:100; phospho-S3-Cofilin (77G2, Cell Signaling), 1:5,000; Rab11 (D4F5, Cell Signaling), 1:500; β-actin (AC-15, Sigma), 1:5,000; γ-actin (2-2.1.14.17, Sigma), 1:5,000; α-tubulin (DM1A, Sigma), 1:1,000; acetylated tubulin (6-11B-1, Sigma), 1:1,000. Secondary antibodies conjugated to FITC, rhodamine (Jackson Immunoresearch), or Alexa Fluor 488/546/647 (Invitrogen) were diluted 1:1,000 (sagittal sections) or 1:200 (whole mount), and samples were incubated 1 h–overnight. F-actin was labelled with phalloidin–Alexa Fluor 546/647 (Invitrogen).
Destrin
Manufactured by Abcam
Sourced in Japan, United Kingdom
Destrin is a protein that binds to and severs actin filaments, playing a key role in the regulation of the actin cytoskeleton. It is commonly used as a research tool in cell biology and biochemistry studies.
Automatically generated - may contain errors
Lab products found in correlation
F actin, by Thermo Fisher Scientific (1 mentions)
α tubulin dm1a, by Merck Group (1 mentions)
G actin, by Merck Group (1 mentions)
Alexa fluor 488 546 647, by Thermo Fisher Scientific (1 mentions)
β actin, by Merck Group (1 mentions)
Keratin 14 prb 155p, by Fortrea (1 mentions)
Prb 440p, by Fortrea (1 mentions)
Limk1, by Abcam (1 mentions)
Immunohistochemistry kit, by Maixin Group (1 mentions)
Vimentin, by Abcam (1 mentions)
E cadherin, by Abcam (1 mentions)
2 protocols using destrin
1
Multi-Antibody Immunofluorescence Imaging
2
Immunohistochemical Analysis of Cytoskeletal Proteins
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The paraffin sections were deparaffinized, followed by hydration, using an immunohistochemistry kit (Maixin Biotech. Co., Fuzhou, China) according to the manufacturer instructions. LIMK1, destrin, E-cadherin, vimentin, Ki-67, and CD34 antibodies (Abcam, Cambridge, UK) were incubated overnight at 4 °C, and normal rabbit immunoglobulin G was considered as a negative control. The specimens were visualized with 3,3′-diaminobenzidine and counterstained with hematoxylin. The staining was brown or tan and located in the cytoplasm or nucleus. The scores according to the degree of positive staining and the percentage of stained cells were as follows: 0, no coloring; 1, light brown; 2, dark brown; 0, staining cells < 5%; 1, staining cells between 5% and 25%; 2, staining cells between 26% and 50%; 3, staining cells > 50%. The score was obtained by adding the intensity and reactivity. The scores < 2 represented low expression, the scores ≥ 2 represented the high expression.
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