Maglumi 4000

All patients underwent a 75 g OGTT and anthropometric measurements. All parameters were measured as previously described [5 (link)].
Fasting blood samples were obtained between the first and fifth day of the menstrual period/withdrawal bleeding. Prolactin, T, FT, and thyroid-stimulating hormone (TSH) were assessed by chemiluminescence immunometric assay (Beckman Unicel DxI 800; Snibe MAGLUMI 4000; Abbott Immulite 2000 analyzer). 17-α-Hydroxyprogesterone was measured using the enzyme-linked immunosorbent assay (ELISA). Plasma glucose was measured using the glucose oxidase method (Hitachi 7600 autoanalyzer). Plasma insulin was measured using a chemiluminescence immunometric assay (Roche e601 analyzer). Total cholesterol (CHOL), TG, HDL-c, and low-density lipoprotein cholesterol (LDL-c) were measured using the enzymatic colorimetric method (Hitachi 7600 autoanalyzer). Indices were calculated based on the following formulas.

  Body mass index (BMI) = weight (kg)/height² (m)

  LAP = (WC (cm)–58) × TG (mmol/L) [9 (link)].

  CVAI = –187.32 + 1.71 × age + 4.23 × BMI + 1.12 × WC (cm) + 39.76 × log10[TG (mmol/L) ] – 11.66 × HDL (mmol/L) [14 (link)].

  VAI = [WC (cm)/[36.58 + (1.89 × BMI)]] × [TG (mmol/L)/0.81] × [1.52/HDL-C (mmol/L)] [15 (link)].

  WTI = WC (cm) × TG (mmol/L) [15 (link)].

  TyG = Ln (fasting triglycerides (mg/dL) × fasting glucose (mg/dL)/2) [17 (link)].

All the recruited patients underwent 75-g OGTT and anthropometric measurements. All parameters were measured, as previously described [7 (link), 22 (link)].
Fasting blood samples were obtained from PCOS patients between the first and fifth day of menstrual period/withdrawal bleeding. Prolactin and total testosterone (TT) were assessed by chemiluminescence immunometric assay (Beckman Unicel DxI 800). Free testosterone (FT) and thyroid-stimulating hormone were also measured using chemiluminescence immunometric assay (Snibe MAGLUMI 4000; Abbott Immulite 2000 analyzer). 17α-hydroxyprogesterone was measured using the ELISA method. Plasma glucose was measured using glucose oxidase method (Hitachi 7600 autoanalyzer). Plasma insulin was measured using chemiluminescence immunometric assay (Roche e601 analyzer). Total cholesterol (CHOL), triglycerides (TG), high-density lipoprotein cholesterol (HDL-c), and low-density lipoprotein cholesterol (LDL-c) were measured using enzymatic colorimetric method (Hitachi 7600 autoanalyzer).