Flexi tube sirna

Manufactured by Horizon Discovery

The Flexi Tube siRNA is a laboratory equipment product designed for RNA interference (RNAi) experiments. It provides a flexible tube format for efficient delivery and transfection of small interfering RNA (siRNA) into cells.

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Lab products found in correlation

Amaxa nucleofector, by Lonza (1 mentions) Cell line nucleofector kit t, by Lonza (1 mentions) Lipofectamine rnaimax kit, by Thermo Fisher Scientific (1 mentions) Opti mem 1, by Thermo Fisher Scientific (1 mentions)

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SiRNAs (161 citations)

2 protocols using flexi tube sirna

Transfection of RBL-2H3 Cells with siRNA

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RBL-2H3
cells were transfected
with Qiagen Flexi Tube siRNA for rat SHIP, SHP-1, and SHP-2 or Lyn
SmartPool siRNA from Dharmacon. The Amaxa Nucleofector and Cell Line
Nucleofector kit T (Lonza Cologne GmbH) were utilized according to
the manufacturer’s protocol. Cells were transfected with 0.25–0.5
μg of siRNA per 1 million cells and plated in regular growth
medium. The cultures were allowed to rest for 24 h before experiments
were performed and the efficiency of knockdown was determined via
immunoblot analyses.

Mahajan A., Barua D., Cutler P., Lidke D.S., Espinoza F.A., Pehlke C., Grattan R., Kawakami Y., Tung C.S., Bradbury A.R., Hlavacek W.S, & Wilson B.S. (2014). Optimal Aggregation of FcεRI with a Structurally Defined Trivalent Ligand Overrides Negative Regulation Driven by Phosphatases. ACS Chemical Biology, 9(7), 1508-1519.

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Targeted Silencing of PTP4A2 and NOTCH1

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The following siRNAs (FlexiTube siRNA) were purchased from Dharmacon: PTP4A2 (SMART Pool, M-009078-01-0005, GE Healthcare Europe GmbH, Dharmacon), NOTCH1 (SMART Pool, L-007771-00-0005, GE Healthcare Europe GmbH, Dharmacon), and negative control (SMART Pool, Non-Targeting siRNA#1, GE Healthcare Europe GmbH, Dharmacon). Transfection was performed using lipofectamine RNAimax kit (Thermofisher Scientific, 13778075) in accordance with the manufacturer’s instructions, with siRNA at a final concentration of 10 nM in Opti-MEM I (Thermofisher scientific, 31985070). Cells were harvested 48 h after transfection.

Poulet M., Sirois J., Boyé K., Uetani N., Hardy S., Daubon T., Dubrac A., Tremblay M.L, & Bikfalvi A. (2020). PRL-2 phosphatase is required for vascular morphogenesis and angiogenic signaling. Communications Biology, 3, 603.

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