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Aav tbg pi cre rbg

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AAV.TBG.PI.Cre.rBG is a recombinant adeno-associated virus (rAAV) vector. It contains a Thyroxine-Binding Globulin (TBG) promoter, a Cre recombinase gene, and a rabbit beta-globin (rBG) polyadenylation signal.

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Lab products found in correlation

Mouse anti flag m2 antibody, by Merck Group (2 mentions) Anti β actin, by Abcam (2 mentions) Aav tbg pi cre rbg, by Addgene (2 mentions) Anti v5 antibody, by Thermo Fisher Scientific (2 mentions) Pcag cre, by Addgene (2 mentions) Mouse pm20d1 flag, by Addgene (2 mentions) Irdye 680rd anti mouse igg, by LI COR (2 mentions) Anti beta tubulin antibody, by Abcam (2 mentions) Anti bhmt, by Abcam (2 mentions) Anti rabbit igg irdye 800rd, by LI COR (2 mentions) Anti biotin streptavidin alexafluor 680, by Thermo Fisher Scientific (2 mentions) Penn aav tmck pi egfp wpre bgh, by Addgene (2 mentions) V5 turboid nes pcdna3, by Addgene (2 mentions) Human fgf1 myc ddk, by OriGene (2 mentions) Human flag fgf2, by Sino Biological (2 mentions) Penn aav tmck pi egfp wpre bgh, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488 anti mouse igg, by Thermo Fisher Scientific (2 mentions)

2 protocols using aav tbg pi cre rbg

1

Comprehensive Antibody and Plasmid Resource

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used: anti-V5 antibody (Invitrogen R960-25), mouse anti-FLAG M2 antibody (Sigma F1804), anti-beta Actin (Abcam ab8227), anti-beta Tubulin antibody (Abcam ab6046), anti-Albumin (Abcam ab19194), anti-BHMT (Abcam 96415), anti-mouse IgG IRDye 680RD (LI-COR 925-68070), anti-rabbit IgG IRDye 800RD (LI-COR 926-32211), anti-biotin Streptavidin AlexaFluor-680 (ThermoFisher S32358), anti-mouse IgG AlexaFluor-488 (Life Technologies A11029-EA. The following plasmids were used: mouse PM20D1-flag (Addgene 84566). pENN.AAV.tMCK.PI.eGFP.WPRE.bGH (Addgene 105556), AAV.TBG.PI.Cre.rBG (Addgene 107787-AAV8), pCAG-Cre (Addgene 13775), V5-TurboID-NES_pCDNA3 (Addgene 107169), human FGF1-myc-DDK (Origene RC207434), human flag-FGF2 (SinoBiological HG10014-NF). ER-TurboID and Mem-TurboID were synthesized (IDT) and inserted into pcDNA3, pENN.AAV.tMCK.PI.eGFP.WPRE.bGH, and AAV.TBG.PI.Cre.rBG expression plasmids with pENTR-d-topo cloning (ThermoFisher K240020) and restriction site cloning. mBHMT-flag was synthesized (IDT) and inserted into pcDNA-DEST40 expression plasmids with pENTR-d-topo cloning. The following adeno-associated viruses (and batch numbers in parentheses) were produced at the Penn Vector Core: AAV8-Tbg-Mem (V7155S), AAV8-Tbg-Cyto (V7119S), AAV8-Tbg-ER (V7069S), AAV9-tMCK-ER (V7074S), AAV8-CB7-FLEx (V7258S).
Wei W., Riley N.M., Yang A.C., Kim J.T., Terrell S.M., Li V.L., Garcia-Contreras M., Bertozzi C.R, & Long J.Z. (2020). Cell type-selective secretome profiling in vivo. Nature chemical biology, 17(3), 326-334.
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2

Comprehensive Antibody and Plasmid Resource

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used: anti-V5 antibody (Invitrogen R960-25), mouse anti-FLAG M2 antibody (Sigma F1804), anti-beta Actin (Abcam ab8227), anti-beta Tubulin antibody (Abcam ab6046), anti-Albumin (Abcam ab19194), anti-BHMT (Abcam 96415), anti-mouse IgG IRDye 680RD (LI-COR 925-68070), anti-rabbit IgG IRDye 800RD (LI-COR 926-32211), anti-biotin Streptavidin AlexaFluor-680 (ThermoFisher S32358), anti-mouse IgG AlexaFluor-488 (Life Technologies A11029-EA. The following plasmids were used: mouse PM20D1-flag (Addgene 84566). pENN.AAV.tMCK.PI.eGFP.WPRE.bGH (Addgene 105556), AAV.TBG.PI.Cre.rBG (Addgene 107787-AAV8), pCAG-Cre (Addgene 13775), V5-TurboID-NES_pCDNA3 (Addgene 107169), human FGF1-myc-DDK (Origene RC207434), human flag-FGF2 (SinoBiological HG10014-NF). ER-TurboID and Mem-TurboID were synthesized (IDT) and inserted into pcDNA3, pENN.AAV.tMCK.PI.eGFP.WPRE.bGH, and AAV.TBG.PI.Cre.rBG expression plasmids with pENTR-d-topo cloning (ThermoFisher K240020) and restriction site cloning. mBHMT-flag was synthesized (IDT) and inserted into pcDNA-DEST40 expression plasmids with pENTR-d-topo cloning. The following adeno-associated viruses (and batch numbers in parentheses) were produced at the Penn Vector Core: AAV8-Tbg-Mem (V7155S), AAV8-Tbg-Cyto (V7119S), AAV8-Tbg-ER (V7069S), AAV9-tMCK-ER (V7074S), AAV8-CB7-FLEx (V7258S).
Wei W., Riley N.M., Yang A.C., Kim J.T., Terrell S.M., Li V.L., Garcia-Contreras M., Bertozzi C.R, & Long J.Z. (2020). Cell type-selective secretome profiling in vivo. Nature chemical biology, 17(3), 326-334.
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