5600 qtof hr ms instrument

Optical rotations were measured on a JASCO P1020 polarimeter (Jasco, Tokyo, Japan) using a 1 cm cell. Ultraviolet (UV) spectra were acquired with a Hitachi U-3010 spectrophotometer (Hitachi High-Technologies, Tokyo, Japan). ECD spectra were recorded on a Chirascan plus CD spectrometer (Applied Photophysics Ltd., Leatherhead, Surrey, UK). IR spectra were recorded on a JASCO 4200 FT-IR spectrometer (Jasco, Tokyo, Japan) using a ZnSe cell. ¹H and ¹³C NMR spectra were measured in DMSO-d₆ or MeOH-d₄ solutions on a Bruker Avance −400, −500, −600, or −800 instrument (Billerica, MA, USA), with solvent peaks at δ_H 2.50/δ_C 39.50 and δ_H 3.31/δ_C 49.00 as their internal standards. High-resolution ESI mass spectrometric data were obtained at the National Instrumentation Center for Environmental Management (Seoul, Korea) and were acquired using an AB Sciex 5600 QTOF HR-MS instrument (Sciex, MA, USA). High-performance liquid chromatography (HPLC) analysis was conducted using a Shimadzu SCL-10A (Shimadzu, Tokyo, Japan) control system connected to a UV–Vis SPD-10A detector (Shimadzu). All solvents used were of spectroscopic grade or distilled from glass prior to use.

Optical rotation was measured with a JASCO P-2000 polarimeter (Easton, MD) with a 1-cm cell. Ultraviolet (UV) spectra were acquired using a Hitachi U-3010 spectrophotometer (Tokyo, Japan). Infrared (IR) spectra were obtained with a JASCO 4200 FT-IR spectrometer (Easton, MD) using a ZnSe cell. High-resolution electrospray ionization mass spectrometry (HR-ESI-MS) data were acquired at the National Instrumentation Center for Environmental Management (Seoul, Korea) using an AB Sciex 5600 QTOF HR-MS instrument (Sciex, MA). Proton and carbon nuclear magnetic resonance spectroscopy (NMR) and two-dimensional NMR spectra were recorded with a Varian Gemini 2000 300 MHz spectrometer (Palo Alto, CA) or Bruker Avance 500 and 600 MHz spectrometers (Berlin, Germany) using MeOH-d₄ with a solvent peak at δ_H 3.31/δ_C 49.0 or dimethyl sulfoxide (DMSO)-d₆ with a solvent peak at δ_H 2.50/δ_C 39.50 as internal standards. High-performance liquid chromatography (HPLC) separation was conducted on a SpectraSYSTEM p2000 instrument equipped with a refractive index detector (SpectraSYSTEM RI-150) and a UV-Vis detector (UV-Vis-151, Gilson, Middleton, WI). All solvents used were of spectroscopic grade or were distilled prior to use.