13xlexaop2 mcd8 gfp

Manufactured by Bloomington Drosophila Stock Center

The 13XLexAOp2-mCD8-GFP is a genetic construct that expresses the membrane-bound form of the green fluorescent protein (mCD8-GFP) under the control of a multimerized LexA operator (13XLexAOp2) promoter. This construct can be used to visualize cellular membranes and study cell morphology in various biological applications.

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Lab products found in correlation

Tub gal80ts, by Bloomington Drosophila Stock Center (1 mentions) Uas myr mrfp, by Bloomington Drosophila Stock Center (1 mentions) R31h09 gal4, by Bloomington Drosophila Stock Center (1 mentions) W1118, by BD (1 mentions) Uas reaper, by BD (1 mentions) Uas tetxlc, by BD (1 mentions) Uas nachbac, by BD (1 mentions) Gmr27e09 gal4, by BD (1 mentions) Mn1 ib gal4, by BD (1 mentions) Uas dsred, by BD (1 mentions) Uas cd8 gfp, by BD (1 mentions)

2 protocols using 13xlexaop2 mcd8 gfp

Drosophila Embryogenesis and Larval Development

Cited 1 time

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Drosophila melanogaster were reared in cages at 25°C, unless indicated otherwise. Embryos were collected onto freshly yeasted apple juice plates overnight and staged according to Campos-Ortega and Hartenstein (1985) . For larval experiments, larvae were picked within 1 h of hatching [designated 0 h after larval hatching (ALH)], transferred to a yeasted food plate and reared to the desired stage before dissection.
The following stocks were used: w¹¹¹⁸, GAL4^c855a (Manseau et al., 1997 (link)), R9D11-mCD8-GFP (Zhu et al., 2011 (link)), R9D11-CD4-tdTomato (Han et al., 2011 (link)), (miR-7)E>GFP (Li et al., 2009 (link)), wg-LacZ (1-en-11) (Kassis et al., 1992), hh^P30 (Lee et al., 1992 (link)) and HLHmγ-GFP (Almeida and Bray, 2005 (link)). The following stocks were obtained from the Bloomington Drosophila Stock Center: dpp-lacZ^Exel.2 (#8411), UAS-myr-mRFP (#7119), R31H09-GAL4 (#49694), R29C07-GAL4 (‘ogre-GAL4’, #49340) and pnt-GFP.FPTB (#42680). To perform FLEXAMP, we crossed flies carrying yw; tub-Gal80^ts, UAS-flp; act>y⁺>LHV2^deltaRFP-86Fb (LexA) (Yagi et al., 2010 (link)) to flies carrying 13XLexAOp2-mCD8-GFP (Bloomington #32205), R31H09-GAL4 and tub-GAL80^ts (Bloomington #7019).

Hakes A.E., Otsuki L, & Brand A.H. (2018). A newly discovered neural stem cell population is generated by the optic lobe neuroepithelium during embryogenesis in Drosophila melanogaster. Development (Cambridge, England), 145(18), dev166207.

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Drosophila Motor Neuron Manipulation

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Drosophila melanogaster were cultured on standard medium at 25°C. Genotypes used in this study include: w 1118 (BDSC 3605); 13X LexAop2-mCD8-GFP (Bloomington Drosophila Stock Center (BDSC) 32204); UAS-DsRed (BDSC 6282); UAS-CD8-GFP (BDSC 32185); UAS-Reaper (BDSC 5823); UAS-TeTXLC (BDSC 28837); UAS-NaChBac (BDSC 9469); GMR27E09-GAL4 (BDSC 49227); MN1-Ib-GAL4 (BDSC 40701); 6-58 Is-GAL4 (Ellie Heckscher, U. Chicago).
Animals of either sex were used depending upon genetic scheme.

Aponte-Santiago N.A., Ormerod K.G., Akbergenova Y., & Littleton J.T. (2020). Synaptic plasticity induced by differential manipulation of tonic and phasic motoneurons in Drosophila.

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