For western blot analysis, the following primary antibodies were used: mouse monoclonal anti-GFAP (ab4648; Abcam, Cambridge, UK), mouse monoclonal anti-Tuj1 (MMS435; Covance, Princeton, NJ, USA), mouse monoclonal anti-P53 (2524; Cell Signaling Technology Inc., Boston, MA, USA), rabbit monoclonal anti-p-CREB (9198; Cell Signaling Technology Inc.), rabbit monoclonal anti-p-PKA (5661; Cell Signaling Technology Inc.) and mouse monoclonal anti-GAPDH (MAB374; Millipore, Billerica, MA, USA).
Mouse monoclonal anti p53
Manufactured by Cell Signaling Technology
Sourced in United States
The Mouse monoclonal anti-p53 is an antibody that specifically binds to the p53 protein. p53 is a transcription factor that plays a crucial role in the regulation of cell cycle, apoptosis, and other cellular processes.
Automatically generated - may contain errors
3 protocols using mouse monoclonal anti p53
1
Immunoblot Analysis of Neural Markers
2
Protein Expression Analysis of Cardiac Progenitor Cells
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Protein lysates of CPCs were obtained using RIPA buffer (Sigma-Aldrich: cat. no. R0278) and protease inhibitors (Torella et al., 2004 (link), Goichberg et al., 2013 (link)). Equivalents of 10 μg proteins were separated on 4–20% SDS-PAGE and subjected to traditional Western blotting. Additionally, equivalents of 1 μg proteins were analyzed with ProteinSimple Wes automated Western blotting system (Harris, 2015 (link)). The following antibodies were utilized: mouse monoclonal anti-p53 (Cell Signaling), rabbit polyclonal anti-p53 (Ser 37) (Cell Signaling Technology: cat. no. 2524, RRID: AB_331743 ), rabbit polyclonal anti-p53 (Ser15) (Cell Signaling Technology: cat. no. 9286S, RRID: AB_331741 ) and mouse monoclonal anti-p16INK4a (Cell Signaling Technology: cat. no. 8884S, RRID: AB_11129865 ). Loading conditions were determined by GAPDH.
3
Cerebellar Protein Expression Analysis
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P15 and P20 WT and pcd mice (n=3/genotype/age) were euthanized and the vermis of the cerebellum was collected and quickly frozen before tissue homogenization in RIPA buffer using GentleMacs® dissociator. 50 g total protein was loaded in electrophoresis gels and immunoblotting was performed. Primary antibodies used were:
rabbit polyclonal anti-CCP1 (1:1000, Proteintech), rabbit polyclonal anti-NCL (1:1000, Abcam), rabbit polyclonal anti-PTEN (1:1000, Abcam), mouse monoclonal anti-p53
(1:500, Cell Signaling) and mouse monoclonal anti-tubulin (1:5000, Sigma).
Values of protein expression were determined using ImageJ software (NIH, USA).
rabbit polyclonal anti-CCP1 (1:1000, Proteintech), rabbit polyclonal anti-NCL (1:1000, Abcam), rabbit polyclonal anti-PTEN (1:1000, Abcam), mouse monoclonal anti-p53
(1:500, Cell Signaling) and mouse monoclonal anti-tubulin (1:5000, Sigma).
Values of protein expression were determined using ImageJ software (NIH, USA).
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