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Interleukin il 1β

Manufactured by Nanjing Jiancheng
Sourced in China

Interleukin (IL)-1β is a cytokine that plays a key role in the inflammatory response. It is involved in the regulation of immune responses, cell proliferation, and differentiation.

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2 protocols using interleukin il 1β

1

Inflammatory Cytokine Quantification

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The PI3K agonist IGF-1 was purchased from Biovision (Milpitas, CA, USA), and a 0.1 mg/ml stock was prepared in saline. Tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and interleukin (IL)-6 rat ELISA kits were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). NGF, TrkA, PI3K, TPRV1, and β-actin antibodies were purchased from Abcam (Cambridge, UK). Akt, p-Akt (Ser473) antibodies, and secondary antibodies goat anti-mouse IgG were obtained from Proteintech (Rosemont, IL, USA). The secondary antibodies goat anti-rabbit IgG was purchased from Huabio (Hangzhou, China).
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2

Oxidative Stress and Inflammation Modulation

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ICA was obtained from Royal pharm, Hanzhou, China. E. coli strain [O55:B5]-derived LPS was acquired from Sigma (St. Louis, MO, USA). ELISA kits of superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), glutathione peroxidase (Gpx)1, myeloperoxidase (MPO), nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-10 were obtained from Nanjing Jiancheng bioengineering institute (Nanjing, China). The primary and secondary antibodies (TLR4, NF-κB pathway proteins and β-actin) were obtained from cell signaling Technology (CST, Beverly, MA, USA).
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