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2 protocols using pip elisa kit

1

Evaluating Cellular Oxidative Stress Responses

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Dulbecco’s modified Eagle medium (DMEM), Ham’s nutrient mixtures medium (F-12 medium), trypsin-EDTA, penicillin-streptomycin (P/S), and fetal bovine serum (FBS) were purchased from Gibco-BRL (Grand Island, NY, USA). PIP ELISA kit was purchased from TaKaRa Bio Inc (Shiga, Japan). The 2,7-dichlorofluorescein diacetate (DCFH2-DA), 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 1,3-Bis (diphenylphosphino) propane (DPPP), diaminofluorophore 4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate (DAF-FM-DA), and the ELISA kits used for the analysis of human MMPs were purchased from Sigma (St. Louis, MO, USA). All other chemicals used in this study were of analytical grade.
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2

Melanogenesis and Oxidative Stress Assays

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Dulbecco’s modified Eagle medium (DMEM), Roswell Park Memorial Institute-1640 (RPMI-1640) medium, Ham’s nutrient mixtures medium (F-12 medium), penicillin-streptomycin (P/S), trypsin-EDTA, and fetal bovine serum (FBS) were purchased from Gibco-BRL (Grand Island, NY, USA). PIP ELISA kit was purchased from TaKaRa Bio Inc (Shiga, Japan). The 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), α-melanocyte-stimulating hormone (α-MSH), acridine orange, 2,2-azobis(2-amidinopropane) hydrochloride (AAPH), 1,3-Bis (diphenylphosphino) propane (DPPP), 2,7-dichlorofluorescein diacetate (DCFH2-DA), diaminofluorophore 4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate (DAF-FM-DA), (Kusatsu, Japan) and the ELISA kits used for the analysis of human matrix metalloproteinases (MMPs) were purchased from Sigma (St. Louis, MO, USA). All other chemicals used in this study were of analytical grade.
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