As previously reported38 (link), 2.5 × 105 cells were plated per well in a 6-well plate. The cells were treated with 1 μmo/L PD0325901 for 48 h, and then collected, pelleted, and resuspended in PBS. Cells were stained with anti-human PD-L1 (Biolegend) and analyzed by flow cytometry. As for the HLA-match analysis, the tumor cells and PBMCs were stained with anti-human HLA-A2 (Biolegend) and analyzed by flow cytometry as previously reported45 (link),46 (link). The Flowjo (Treestar) software was used for the analysis of flow cytometry data. The standardized fluorescence intensities were calculated by dividing the median fluorescence intensities of specific antibodies by the median fluorescence intensities of isotype controls. The results are presented as mean ± SD of three independent experiments.
Anti human hla a2
Manufactured by BioLegend
Sourced in United States
Anti-human HLA-A2 is a biomarker that specifically recognizes and binds to the HLA-A2 allele expressed on the surface of human cells. It is commonly used in research applications for the identification and characterization of HLA-A2-positive cells.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti human hla a2
1
Quantifying PD-L1 and HLA-A2 Expression
2
Characterization of CRT and HLA-A2 Expression
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CRC cells and PBMCs were collected and suspended in PBS. All cells were incubated with anti-human CRT (Biolegend, San Diego, CA, USA) or anti-human HLA-A2 (Biolegend, USA) for 30 min. The single-cell suspensions of mouse spleen were processed by mashing the spleen through 40 μm cell strainers and then incubated in RBC lysis buffer (Sigma-Aldrich, St Louis, MO, USA). Samples were detected on a CytoFLEX cytometer (Beckman Coulter, Germany). The antibodies: CD8 (clone 53-6.7), CD3 (clone 3C7), CD4 (clone IM7) were obtained from Biolegend (USA). The Flowjo software (Treestar, Woodburn, OR, USA) was used to analyze flow cytometry data.
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