Cells were rinsed twice in ice-cold phosphate-buffered saline (PBS) and fixed with a freshly prepared solution of 4% paraformaldehyde in PBS for 20 minutes and permeabilized for 10 minutes with 0.1% digitonin in PBS. After several rinses in ice-cold PBS, cells were incubated in 3% normal goat serum in PBS (blocking solution) for 30 minutes at room temperature, followed by an overnight incubation at 4°C with primary antibodies. Cells were extensively washed with PBS-0.1% digitonin and then incubated with Alexa-conjugated secondary antibodies (Molecular Probes) for 60 minutes at room temperature. Coverslips were mounted in a mounting medium. Images were captured with an API Delta Vision deconvolution microscope using SoftWorx software (Applied Precision, Issaquah, Washington, USA). Primary antibodies used were anti-AR (Sc-816, 1:200, Santa Cruz Biotechnology Inc.), anti-VAMP7 (NB100-91356, 1:1,000, Novus Biologicals), ESR1 (MA1-310, 1:200, Thermo-Fisher) and anti-RAB5 (#2143, 1:100, Cell Signaling).
Esr1 ma1 310
Manufactured by Thermo Fisher Scientific
The ESR1 (MA1-310) is a lab equipment product offered by Thermo Fisher Scientific. It is used for the detection and quantification of the estrogen receptor alpha (ER-alpha) protein. The core function of this product is to facilitate the analysis of ER-alpha levels in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Api delta vision, by Cytiva (2 mentions)
Anti vamp7 nb100 91356, by Novus Biologicals (2 mentions)
Softworx, by Cytiva (2 mentions)
Anti rab5, by Cell Signaling Technology (2 mentions)
Anti ar sc 816, by Santa Cruz Biotechnology (2 mentions)
Alexa conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions)
2 protocols using esr1 ma1 310
1
Immunofluorescence Staining of Cellular Proteins
2
Immunofluorescence Staining of Cellular Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were rinsed twice in ice-cold phosphate-buffered saline (PBS) and fixed with a freshly prepared solution of 4% paraformaldehyde in PBS for 20 minutes and permeabilized for 10 minutes with 0.1% digitonin in PBS. After several rinses in ice-cold PBS, cells were incubated in 3% normal goat serum in PBS (blocking solution) for 30 minutes at room temperature, followed by an overnight incubation at 4°C with primary antibodies. Cells were extensively washed with PBS-0.1% digitonin and then incubated with Alexa-conjugated secondary antibodies (Molecular Probes) for 60 minutes at room temperature. Coverslips were mounted in a mounting medium. Images were captured with an API Delta Vision deconvolution microscope using SoftWorx software (Applied Precision, Issaquah, Washington, USA). Primary antibodies used were anti-AR (Sc-816, 1:200, Santa Cruz Biotechnology Inc.), anti-VAMP7 (NB100-91356, 1:1,000, Novus Biologicals), ESR1 (MA1-310, 1:200, Thermo-Fisher) and anti-RAB5 (#2143, 1:100, Cell Signaling).
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